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Western blot - Aldolase antibody (ab54770)
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Immunocytochemistry/ Immunofluorescence - Aldolase antibody (ab54770)
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Product Name
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Aldolase antibody
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See all Aldolase antibodies (11)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to Aldolase
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Immunogen
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Recombinant full length protein, corresponding to amino acids 1-365 of Human Aldolase
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Reacts with
(species key)
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Hu
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.22 - 0.50 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Aldolase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Aldolase antibody images:
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 Western blot - Aldolase antibody (ab54770)
Predicted band size : 39 kDa
Aldolase antibody (ab54770) at 1ug/lane + A-431 cell lysate at 25ug/lane.
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Immunocytochemistry/ Immunofluorescence - Aldolase antibody (ab54770)
ab54770 at 10 ug/ml staining Aldolase in human Hela cells by Immunocytochemistry / Immunofluorescence.
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References for Aldolase antibody (ab54770)
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This product has been used in:
Srensen BS et al. Proteins upregulated by mild and severe hypoxia in squamous cell carcinomas in vitro identified by proteomics. Radiother Oncol 92:443-9 (2009). WB; Human. PubMed: 19541378
If you publish research using ab54770 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Aldolase
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