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Anti-Aldolase antibody
See all Aldolase products (11) ...
Goat polyclonal to Aldolase
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, purified and partially purified Aldolase from rabbit muscle. Detects human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.
WB, IP, ELISAmore details
Reacts with
Rabbit, Human
Aldolase from rabbit muscle
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
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IgG fraction
Prepared from monospecific antiserum by a delipidation, slat fractionation and ion exchange chromatography.
Polyclonal
IgG
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Western blot - Aldolase antibody (ab6190)
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Our Abpromise guarantee covers the use of ab6190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration (from PMID 17611631).
Suggested working dilution(s):
against 1.0 µg of Aldolase from rabbit muscle in standard sandwich ELISA - 1/6000 to 1/26,000
Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate.
Optimal titers for other applications should be determined by the researcher.
This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.
Cytoplasmic
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