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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Aldolase antibody
See all Aldolase products (11) ...
Description
Goat polyclonal to Aldolase
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, purified and partially purified Aldolase from rabbit muscle. Detects human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.
Tested applications
WB, IP, ELISAmore details
Cross reactivity
Reacts with
Rabbit, Human
Immunogen
Aldolase from rabbit muscle
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Prepared from monospecific antiserum by a delipidation, slat fractionation and ion exchange chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
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Western blot - Aldolase antibody (ab6190)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
ELISA
Application notes
WB: Use at an assay dependent concentration (from PMID 17611631).
Suggested working dilution(s):
against 1.0 µg of Aldolase from rabbit muscle in standard sandwich ELISA - 1/6000 to 1/26,000
Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate.
Optimal titers for other applications should be determined by the researcher.
Target
Relevance
This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.
Cellular localization
Cytoplasmic
Alternative names
- ALDA antibody
- ALDOA antibody
- Aldolase A antibody
see all
Database links
- Entrez Gene: 226 Human
- Omim: 103850 Human
- SwissProt: P04075 Human
- SwissProt: Q6FI10 Human
- SwissProt: P00883 Rabbit
- Unigene: 513490 Human
Anti-Aldolase antibody images:
References for Anti-Aldolase antibody (ab6190)
This product has been referenced in:
- O'Connell Ket al. Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle. Int J Mol Med 20:145-53 (2007).Read more (PubMed: 17611631) »
See 1 publication for this product
Publishing research using ab6190? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"