Aldolase antibody (ab6190)

Product Name 

Aldolase antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to Aldolase

Immunogen 

Aldolase from rabbit muscle

Reacts with 
(species key)

Hu, Rb

Specificity 

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, purified and partially purified Aldolase from rabbit muscle.

Detects human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.

Tested applications 
(see key)

ELISA, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: Use at an assay dependent concentration (from PMID 17611631).
Suggested working dilution(s):
against 1.0 µg of Aldolase from rabbit muscle in standard sandwich ELISA - 1/6000 to 1/26,000
Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate.
Optimal titers for other applications should be determined by the researcher.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Prepared from monospecific antiserum by a delipidation, slat fractionation and ion exchange chromatography.

Form 

Liquid

Concentration 

10.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Aldolase antibody (ab6190)

  This product has been used in: (two most recent references)

O'Connell K et al. Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle. Int J Mol Med 20:145-53 (2007). PubMed: 17611631

Zaman K et al. Protection from oxidative stress-induced apoptosis in cortical neuronal cultures by iron chelators is associated with enhanced DNA binding of hypoxia-inducible factor-1 and ATF-1/CREB and increased expression of glycolytic enzymes, p21(waf1/cip1), and erythropoietin. J Neurosci 19:9821-30 (1999). PubMed: 10559391
If you publish research using ab6190 please let us know so that we can cite the reference on this datasheet.

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