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|Sample type||Average %||Range|
|Urine||= 98.7||7.5pg/ml - 120pg/ml|
|Serum||= 69.8||7.5pg/ml - 120pg/ml|
|Plasma||= 108.3||7.5pg/ml - 120pg/ml|
Abcam’s Aldosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Aldosterone in plasma, serum and urine.
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Aldosterone antigen and a polyclonal sheep antibody specific to Aldosterone. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Aldosterone captured in the plate.
|Compound||% Cross Reactivity|
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 27ml|
|Aldosterone Alkaline Phosphatase Conjugate||1 x 5ml|
|Aldosterone Antibody||1 x 5ml|
|Aldosterone Standard||1 x 250µl|
|Assay Buffer||1 x 27ml|
|Donkey anti-sheep IgG Microplate (12 x 8 wells)||1 unit|
|Plate Sealer||1 unit|
|pNpp Substrate||1 x 20ml|
|Stop Solution||1 x 5ml|
Our Abpromise guarantee covers the use of ab136933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
Aldosterone measured in various samples showing quantity (pg) per mL of sample tested.
Representative standard curve using ab136933.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"