Overview

  • Product name
    Aldosterone ELISA kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Aldosterone 20 84.8pg/ml 4.5%
    Aldosterone 20 27.7pg/ml 4.4%
    Aldosterone 20 13.9pg/ml 6.6%
    Inter-assay
    Sample n Mean SD CV%
    Aldosterone 13 72.6pg/ml 10.8%
    Aldosterone 13 24.4pg/ml 18%
    Aldosterone 13 10.8pg/ml 16.3%
  • Sample type
    Urine, Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 4.7 pg/ml
  • Range
    3.9 pg/ml - 250 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine = 98.7 7.5pg/ml - 120pg/ml
    Serum = 69.8 7.5pg/ml - 120pg/ml
    Plasma = 108.3 7.5pg/ml - 120pg/ml

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Mammal
  • Product overview

    Abcam’s Aldosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Aldosterone in plasma, serum and urine.

    A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Aldosterone antigen and a polyclonal sheep antibody specific to Aldosterone. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Aldosterone captured in the plate.

  • Notes
    Compound% Cross Reactivity

    11-Deoxycorticosterone

    0.30%

    Progesterone

    0.20%

    Corticosterone

    0.19%

    Cortisol

    ≤ 0.001%

    DHT

    ≤ 0.001%

    Estradiol

    ≤ 0.001%

    Testosterone

    ≤ 0.001%

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab136933 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Competitive ELISA Use at an assay dependent concentration.

    Aldosterone ELISA kit images

    • Aldosterone measured in various samples showing quantity (pg) per mL of sample tested.

    • Representative standard curve using ab136933.

    Protocols

    References for Aldosterone ELISA kit (ab136933)

    This product has been referenced in:
    • Lee CT  et al. The role of calbindin-D28k on renal calcium and magnesium handling during treatment with loop and thiazide diuretics. Am J Physiol Renal Physiol 310:F230-6 (2016). Read more (PubMed: 26582761) »
    • Joshi SR  et al. Cyp2c44 gene disruption exacerbated pulmonary hypertension and heart failure in female but not male mice. Pulm Circ 6:360-8 (2016). Read more (PubMed: 27683613) »

    See all 3 Publications for this product

    Product Wall

    human cell culture supernatant

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    I've used this assay kit to measure aldosterone production in cell culture supernatant of a H295R human adrenal gland carcinoma cell line. This kit gives reliable values for serial dilutions of samples. The performance of the assay was not influenced by the use of medium instead of assay buffer with a minimum dilution of 1:8.
    This is a reliable product to measure aldosteron values in cell culture supernatants.
    Username

    Miss. Saskia Scheij

    Verified customer

    Submitted Oct 31 2013

    Hello! Please find the reply from the lab below:

    The TA (Total Activity) well is a control that is included in our competitive assays to indicate whether or not the conjugate is working as expected. If the TA value, for example, was normal bu...

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    Unfortunately, we have only validated this assay for plasma, serum and urine. We therefore do not know the best way of preparing the samples nor do we have any tissue related citations for this assay.

    Aldosterone itself is a species ind...

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    Thank you very much for your inquiry and your interest in our AbTrial programme.


    I am very pleased to hear you would like to test ab138875 with mouse serum, mouse urine and tissue culture supernatant. This provided code will give you &pou...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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