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Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)

Q&A (43)Specific References (5)

Overview

  • Product nameAlkaline Phosphatase Assay Kit (Colorimetric)See all Alkaline phosphatase kits ...
  • Tests
    500 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 10 µU
  • Range
    10 µU - 250 µU
  • Product overview

    Abcam's Alkaline Phosphatase Assay Kit (Colorimetric) is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure ALP activity in serum and biological samples. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (?max= 405 nm) when dephosphorylated by ALP.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This kit contains 10 substrate tablets providing convenience for multiple usages.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceAlkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone.
  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
    • Alternative names
        Alkaline phosphatase intestinalAlkaline phosphatase liver/bone/kidneyAlkaline phosphatase liver/bone/kidney isozyme
        Alkaline phosphatase tissue nonspecific isozymeAlkaline phosphomonoesteraseALPIALPLAP TNAPGlycerophosphataseHOPSIAPIntestinal alkaline phosphataseKasahara isozymeLiver/bone/kidney type alkaline phosphataseTissue nonspecific ALPTNAPTNSALP
      see all

    Applications

    Our Abpromise guarantee covers the use of ab83369 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Alkaline Phosphatase Assay Kit (Colorimetric) images

    • Functional Studies - Alkaline Phosphatase Colorimetric Assay Kit (ab83369)
      Functional Studies - Alkaline Phosphatase Colorimetric Assay Kit (ab83369)
      p-Nitrophenol (pNP) standard curve.

    Protocols

    References for Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)

    This product has been referenced in:
    • Rajakumar A  et al. Transcriptionally active syncytial aggregates in the maternal circulation may contribute to circulating soluble fms-like tyrosine kinase 1 in preeclampsia. Hypertension 59:256-64 (2012). Read more (PubMed: 22215706) »
    • Nepelska M  et al. Butyrate produced by commensal bacteria potentiates phorbol esters induced AP-1 response in human intestinal epithelial cells. PLoS One 7:e52869 (2012). Functional Studies ; Human . Read more (PubMed: 23300800) »

    See all 5 Publications for this product

    Publishing research using ab83369 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab83369 Submit a Question for ab83369

    Displaying 1 - 10 of 43 results for Abreviews and Q&A

    10
    Votes: Highest First

    Regarding ab83369, does the assay buffer facilitate cell lysis - is it a lysis buffer?
    Our customer plans to work with adherent cells with each well containing different cell numbers, is there a way to to measure AP activity without detaching the cel...

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    The composition information of the lysis buffer is proprietary so I am unable to release it for your customer. However, I can confirm that the assay buffer will indeed help in lysing the cells since it contains some mild detergent.

    Standard in Kit ab83369

    Vielen Dank für Ihren Anruf.

    Ich habe im Labor nachgefragt und kann Ihnen nun bestätigen, dass der Standard nicht wirklich in Units angegeben wird sonder als eine Menge.

    Mittels des Standards kann man die Menge an pNP in den ...

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    Our customer received Alkaline phosphatase colorimetric assay kit (Abcam) that will expire in January (lot #GR49258-1).
    The customer wonders if it may be used after its expiration date.

    Thank you for your enquiry.
    We guarantee all of our products to work for 6 months from the date of purchase when stored correctly.
    As indicated in the protocol booklet, the kit must be stored at -20ºC in the dark. Please note the enzymes are sta...

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    We have received an enquiry from a customer, please see below;

    I am using ALP assay Kit (colorimetric) (ab83369). However, I run out of ALP Assay buffer, pNPP substrate and stop solution. I wonder if I could buy them from you and how much they co...

    Read More

    Thank you for contacting us.

    Unfortunately these separate components will cost you more than the cost of kit. So I would suggest to buy the whole kit again. I am sorry for inconvenience but is in your better interest.

    I hope this informa...

    Read More

    Needs a kit that can detect SEAP in serum samples.

    Thank you very much for your call last week and for your patience while I've been in touch with the lab regarding your enquiry.

    We don't have any data to indicate that ab83369 has been used to detect SEAP or that ab133077 has been used with seru...

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    Customer kindly contacted us to inquire about sample background controls with use of this product.

    Thank you for contacting us.

    I have contacted the lab and received reply regarding your questions. While we always recommend using controls for each sample to eliminate any background interference, if the samples are colorless you may skip th...

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    Hi Abcam,

    Please see below for an email we have received from a customer;

    I have a question about how to get cells homogenized when using ALP kit (ab83369) to measure intracellular ALP. For my samples before test, cells are attached ...

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    Thank you for your enquiry regarding ab83369.

    The cells can be homogenized exactly as the customer suggested. We would suggest adding the ALP assay buffer to the adherent cells; scraping the cells in the assay buffer, taking all the lysate in a ...

    Read More

    Hello,

    I would like to ask about possibility to assay Alkaline Phosphatase using ab83369 from murine cell line?

    Thank you in advance.

    Thank you for your enquiry.

    I can confirm have tested ab83369 Alkaline Phosphatase Assay Kit (Colorimetric) with rabbit serum. But since the priniciple is the same, the kit should work in all mammalian samples including mouse samples. Here is a...

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    Can I buy the buffer for ab83369 separately?

    Thank you for contacting us.

    The buffer for ab83369 is not available for purchase separately. You should have received 100 mL of buffer with the kit, which should be more than enough to run 500 tests. If you did not receive the correct volume, w...

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    Regarding ab83369 ,I know it's usually not possible but the customer has asked if it is possible to get small samples of the three batch kits to check they work for him.

    Also, if the customer orders one kit, is it possible to reserve 2 batch fo...

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    Thank you for your reply which has been forwarded to me as my colleague Anja is currently away from the office.

    I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible for us to stock small sample sizes ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"