Overview

  • Product nameAlkaline Phosphatase Assay Kit (Fluorometric)
    See all Alkaline phosphatase kits
  • Tests
    500 x 1 test
  • Sample type
    Saliva, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    In Abcam's Alkaline Phosphatase Fluorometric Assay Kit, ALP cleaves the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (MUP) substrate resulting in an intense fluorescent signal (Ex/Em = 360nm/440nm). The kit is an ultra sensitive, simple, direct and HTS-ready assay designed to measure ALP activity in serum and bio-samples with detection sensitivity ~1 µU, more sensitive than colorimetric assays.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. The change in alkaline phosphatase level and activity is associated with a lot of diseases in the liver and bones. Alkaline phosphatase is also a popular enzyme conjugated to secondary antibody in ELISA.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceAlkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone.
  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
  • Alternative names
    • Alkaline phosphatase intestinal
    • Alkaline phosphatase liver/bone/kidney
    • Alkaline phosphatase liver/bone/kidney isozyme
    • Alkaline phosphatase tissue nonspecific isozyme
    • Alkaline phosphomonoesterase
    • ALPI
    • ALPL
    • AP TNAP
    • Glycerophosphatase
    • HOPS
    • IAP
    • Intestinal alkaline phosphatase
    • Kasahara isozyme
    • Liver/bone/kidney type alkaline phosphatase
    • Tissue nonspecific ALP
    • TNAP
    • TNSALP
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab83371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Alkaline Phosphatase Assay Kit (Fluorometric) images

  • 4-MU measured in mouse tissue lysates showing quantity (mU) per mg protein of tested sample

  • 4-methylumbelliferyl measured in biological fluids showing quantity (mU) per microliter of tested sample

  • 4-Methylumbelliferone (4-MU) standard curve.

Protocols

References for Alkaline Phosphatase Assay Kit (Fluorometric) (ab83371)

This product has been referenced in:
  • Yoo BK  et al. Human intestinal epithelial cell line SK-CO15 is a new model system to study Na(+)/H(+) exchanger 3. Am J Physiol Gastrointest Liver Physiol 303:G180-8 (2012). Human . Read more (PubMed: 22556145) »

See 1 Publication for this product

Product Wall



This kit is actually very sensitive, with a detection sensitivity ˜1 µU, which is more sensitive than colorimetric assays.

Since your standards worked, I do not suspect that there is a problem with the kit or your handling of ...

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Thank you for your enquiry regarding ab83371.

This is the buffer we recommend in our protocol to homogenize the cells/tissues, so it is used in lieu of the lysis buffer. It will not interfere with the BCA quantification.

If you need...

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For Acid Phosphatase Assay Kit ab83370, the pH of the buffer prevents alkaline phosphatase from working (ALP can only function in an alkaline pH whereas AP works in an acidic pH) so you only measure AP activity.
Acid phosphatase (AP) can depho...

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Hello and thank you for contacting us. The lyophilzed ALP enzyme should be stored at -20. Once you reconstitute the enzyme you should not freeze the solution. Please let me know if you have any further questions.

Thank you for your reply which has been forwarded to me as my colleague Anja is currently away from the office.

I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible for us to stock small sample siz...

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Thank you for your enquiry and your interest in our products.

This kit (ab83371: Alkaline Phosphatase Assay Kit (Fluorometric)) has only been characterized on human sample; other species have not been tested yet. However, this is an enzyme-ba...

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Thank you for your inquiry.

I am happy to confirm that we do have three different lots for ab83369 at this moment in time. Unfortunately, we only have one lot in stock at the moment for ab83371.

Please inform your customer that our ...

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Regarding the alkaline phosphatase assay that gave unusually high readings, could you please tell us how you diluted the substrate before use in the standards and samples? The protocol instructions are fairly straightforward, but we just want to be sur...

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Thank you for confirming these details.

I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of char...

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Thank you for bringing this to our attention. To help us understand the problem, I would like to ask a few questions.

You mentioned that that only the zero standard was within the limits of the plate reader's range. This was the well that di...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"