You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)]
See all Alkaline Phosphatase, Tissue Non-Specific products (7) ...

Description

Mouse monoclonal [3H414 (TRA-2-49)] to Alkaline Phosphatase, Tissue Non-Specific

Tested applications

IHC-P, IF, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Rabbit, Cat, Human, Pig, Chimpanzee, Gorilla, Hoolock Gibbon, Orangutan, Owl monkey

Does not react with

Mouse, Rat, Sheep, Goat, Guinea pig, Hamster, Cow, Dog, Baboon, Rhesus monkey

Immunogen

2102Ep Human Embryonal Carcinoma Cells

General notes

Clone 3H414 was previously known as TRA-2-49/6E.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.6

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

3H414 (TRA-2-49)

Isotype

IgG1

Research areas

Tags & Cell Markers >> Fusion / Marker Proteins >> Alkaline Phosphatase
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases

  • Immunocytochemistry/ Immunofluorescence - Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973)Immunocytochemistry/ Immunofluorescence - Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab17973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

This isozyme may play a role in skeletal mineralization.

Involvement in disease

Defects in ALPL are a cause of hypophosphatasia (HOPS) [MIM:146300]. HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto).
Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC) [MIM:241510].
Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI) [MIM:241500].

Sequence similarities

Belongs to the alkaline phosphatase family.

Post-translational
modifications

Glycosylated.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionP05186 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AKP2 antibody
  • Alkaline phosphatase liver/bone/kidney antibody
  • Alkaline phosphatase liver/bone/kidney isozyme antibody
  • Alkaline phosphatase tissue nonspecific isozyme antibody
  • Alkaline phosphatase, tissue-nonspecific isozyme antibody
  • Alkaline phosphomonoesterase antibody
  • ALPL antibody
  • AP TNAP antibody
  • AP-TNAP antibody
  • APTNAP antibody
  • BALP antibody
  • BAP antibody
  • FLJ40094 antibody
  • FLJ93059 antibody
  • Glycerophosphatase antibody
  • HOPS antibody
  • Liver/bone/kidney isozyme antibody
  • Liver/bone/kidney type alkaline phosphatase antibody
  • MGC161443 antibody
  • MGC167935 antibody
  • PHOA antibody
  • PPBT_HUMAN antibody
  • Tissue non specific alkaline phosphatase antibody
  • Tissue nonspecific ALP antibody
  • TNAP antibody
  • TNSALP antibody
see all

Database links

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] images:

  Immunocytochemistry/ Immunofluorescence - Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973)

Immunocytochemistry/ Immunofluorescence - Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973)

Undifferentiated human embryonic stem cells (H9 line) were stained with the tissue non-specific alkaline phosphatase antibody, ab17973. The cells were positive for alkaline phosphatase with staining localised on the surface membrane.

Dilution used: 1/50

Note that the feeder cells in the bottom left hand corner of the plate are not stained by this antibody.

Ludovic Vallier, University of Cambridge

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973)

ab17973 staining Alkaline Phosphatase, Tissue Non-Specific in human adrenal gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated rabbit anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.

This image is courtesy of an Anonymous Abreview

See Abreview

References for Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973)

This product has been referenced in:

  • Kemp K  et al. Inflammatory cytokine induced regulation of superoxide dismutase 3 expression by human mesenchymal stem cells. Stem Cell Rev 6:548-59 (2010).Read more (PubMed: 20683679) »
  • Liu Y  et al. Periodontal ligament stem cell-mediated treatment for periodontitis in miniature swine. Stem Cells 26:1065-73 (2008). ICC/IF; Pig.Read more (PubMed: 18238856) »

See all 3 publications for this product

Publishing research using ab17973? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"