Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
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ab354 has been referenced in 3 publications.
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Ab354 staining Alkaline Phosphatase in E.coli recombinant protein Mach1 by indirect ELISA. Samples were blocked with 5%milk for 3 hours at room temperature and incubated with primary antibody (1/15000 in TBST + 5% Milk) for 90 minutes. An HRP-conjugated goat polyclonal to Rabbit IgG (1/30000) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
All lanes : Anti-Alkaline Phosphatase antibody (ab354) at 1/8000 dilution
Lane 1 : Escherichia coli Recombinant protein (Mach1)
Lane 2 : Escherichia coli Recombinant protein (Mach1)
Lysates/proteins at 0.5 µg per lane.
Secondary
An HRP-conjugated anti-rabbit IgG (whole molecule) at 1/16000 dilution
developed using the ECL technique
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 seconds
Image courtesy of an anonymous Abreview.
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