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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Overview
Product name
Anti-Alkaline Phosphatase antibody
See all Alkaline Phosphatase products (10) ...
Description
Rabbit polyclonal to Alkaline Phosphatase
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli].
Tested applications
WB, IP, ELISAmore details
Cross reactivity
Reacts with
Escherichia coli
Does not react with
all Mammals
Immunogen
Full length protein (E. coli).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Purity
Whole antiserum
Purification notes
This product was prepared from monospecific antiserum by a delipidation and defibrination.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> Phosphatases
Tags & Cell Markers >> Fusion / Marker Proteins >> Alkaline Phosphatase
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/8000 - 1/40000.
IP: Use at an assay dependent dilution.
ELISA: 1/8000 - 1/40000.
Target
Relevance
Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Cellular localization
Cell Membrane; lipid anchor; GPI anchor.
Alternative names
- GCAP antibody
- Germ cell alkaline phosphatase antibody
- Nagao isozyme antibody
see all
Anti-Alkaline Phosphatase antibody images:
ELISA - Alkaline Phosphatase antibody (ab354)
Ab354 staining Alkaline Phosphatase in E.coli recombinant protein Mach1 by indirect ELISA. Samples were blocked with 5%milk for 3 hours at room temperature and incubated with primary antibody (1/15000 in TBST + 5% Milk) for 90 minutes. An HRP-conjugated goat polyclonal to Rabbit IgG (1/30000) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot - Alkaline Phosphatase antibody (ab354)
All lanes : Anti-Alkaline Phosphatase antibody (ab354) at 1/8000 dilution
Lane 1 : Escherichia coli Recombinant protein (Mach1)
Lane 2 : Escherichia coli Recombinant protein (Mach1)
Lysates/proteins at 0.5 µg per lane.
Secondary
An HRP-conjugated anti-rabbit IgG (whole molecule) at 1/16000 dilution
developed using the ECL technique
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 seconds
Image courtesy of an anonymous Abreview.
References for Anti-Alkaline Phosphatase antibody (ab354)
This product has been referenced in:
- Downer Ret al. The elastin-binding protein of Staphylococcus aureus (EbpS) is expressed at the cell surface as an integral membrane protein and not as a cell wall-associated protein. J Biol Chem 277:243-50 (2002).Read more (PubMed: 11684686) »
- Bross Pet al. The HSP60-(P.val98ile) mutation associated with hereditary spastic paraplegia SPG13 compromises chaperonin function both in vitro and in vivo. J Biol Chem : (2008). WB; Escherichia coli .Read more (PubMed: 18400758) »
See all 3 publications for this product
Publishing research using ab354? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"