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Alkaline Phosphatase chromogen (BCIP/NBT) - Ready to Use (ab7468)

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Overview

  • Product nameAlkaline Phosphatase chromogen (BCIP/NBT) - Ready to UseSee all Alkaline Phosphatase Chromogen reagents ...
  • Tested applicationsWB, Dot Blot, IHC-P, IHC-Fr more details
  • General notesBCIP: 5-bromo-4-chloro-3-indolyl phosphate NBT: p-nitroblue tetrazolium chloride This product used to be called "Alkaline phosphatase detection kit (BCIP/NBT)". Abcam has a wide range of primary and secondary antibodies available on the Abcam website to suit your protocol needs.

    • Properties

  • Storage instructionsStore at +4°C.
  • Storage bufferpH: 9.80
    Constituents: BCIP, NBT
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab7468 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration.

    BCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light.
    Dot Blot Dot: Use at an assay dependent concentration.
    IHC-P IHC-P: Use at an assay dependent concentration.
    IHC-Fr IHC-Fr: Use at an assay dependent concentration.

    References for Alkaline Phosphatase chromogen (BCIP/NBT) - Ready to Use (ab7468)

    ab7468 has not yet been referenced specifically in any publications.

    Publishing research using ab7468 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab7468 Submit a Question for ab7468

    Displaying 1 - 10 of 19 results for Abreviews and Q&A

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    I would like to know if I can receive a free sample for ab7468 (Alkaline Phosphatase chromogen (BCIP/NBT) - Ready to Use) to try it out. I did use other products in the past but had some minor problems. Taking into account that I am using the secondary Ab...

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    Thank you for contacting us. Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products. We only have the 100mL size available for ab7468.

    We are happy to reassure our customers that all of our p...

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    Inquiry: is it suitable to use with AP conjugated secondary antibody ?

    Thank you for your enquiry.

    I am sorry to confirm that ab133406 is not suitable for detection of AP. This detection system has been designed for optimal detection of HRP, and will not be as sensitive for detection of AP.

    I am sorry I...

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    To technical support,



    I would like to order a complete set of antibodies and controls from Abcam for WB analysis. Could you check if these antibodies would suit for the analysis. In addition, could you recommend some positive contro...

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    Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
    I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab24102....

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    Dear Technical Team

    Our customer boutht ab7468, BCIP/NBP ready to use.
    She will use as a westerblot substrate.
    Could let me know specific protocol?
    For ex, Does she have to dilute?
    How long she need incubation time. How to w...

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    Thank you for your inquiry.

    I can confirm that the ab7468 Alkaline Phosphatase chromogen (BCIP/NBT)substrate is ready to use:

    Wash the membrane after incubation with the alkaline phosphatase secondary.
    Apply sufficient substrate...

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    I have written out the assay method for immunohistochemical determination of MMP1. Would you be able to review it and give me your comments? Please feel free to edit it if it is required.

    Thank you for your reply.

    I looked over the protocol that you sent and it looks to be OK with me. I had a colleague look at it aswell and they thought it was good too.

    If there is anything else I can help you with, or if you have any f...

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    IHC-Fr protocol and reagent to be used with ab4667. DAB and H7E subsrate quote aswell.

    Thank you for calling Abcam earlier today.

    To be able to send a quote for the products you requested, please could you send me your shipping and billing address.

    Once I get this information, I can send that quote out.

    Also fo...

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    Inquiry: I am looking to use this kit with an AP-conjugated secondary. We currently use HRP secondaries in a SynGene dark-box to detect the chemiluminescence. Would this product be suitable for detection in the same dark-box as there is an option to have ...

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    Thank you for contacting us.

    NBT/BCIP system produces a colorimetric substrate that yields an intense and insoluble black-purple precipitate when reaction with alkaline phosphatase.

    Unlike HRP chemiluminiscent methods, the chromogenic ...

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    I repeated the experiment and diluted plasma samples out to 1:4800 fold but this dilution dependent signal increase phenomenon persisted. In other words, the signal from the 1:4800 fold dilution was higher than the 1:2 dilution! I really doubt if this is ...

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    I am not aware of any cross reactivity of this antibody with human Ig. However we do offer a number of secondary antibodies which are designed to reduce background levels by either being pre-absorbed against other proteins and Igs or by digestion into F(a...

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    Many thanks for your reply. I really appreciate your suggestions. My detection is working well now. However I am facing some strange results which I would love your thoughts on please:   I am struggling to interpret an indirect ELISA I am making. It inv...

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    Thank you for contacting me again. It is always a pleasure to hear from you.

    After reviewing your email, I am lead to believe that this really is a hook effect as you had mentioned. I am not an expert on this hook effect in plasma specifically,...

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    Thanks for your advice and suggestions last time. I got another ab7468 and it is working well, both for WB and my reattempt ELISA.   However I have a question which I hope you may have an answer to:   After adding the pNPP substrate, I waited for 30 m...

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    Thank you for contacting us, I am glad to hear that the new product is working well for you.

    Usually when color is developing slowly it is one of three issues. 1) the plates and reagents have not come to room temperature. 2) the conjugates are ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"