Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit (ab108854)
- Product nameAlpha 1 acid Glycoprotein (AGP) Human ELISA KitSee all Alpha 1 acid Glycoprotein kits ...
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity= 0.06 µg/ml
- Range0.063 µg/ml - 4 µg/ml
= 98.5 %
- Assay time3h 00m
- Species reactivityReacts with: Human
- Product overview
Alpha 1 acid Glycoprotein (AGP) is an acute-phase protein secreted by the liver which under conditions of inflammation increase several-fold in concentration. An elevated serum level of acute-phase inflammatory markers is associated with an increased risk of cardiovascular disease Urinary orosomucoid excretion rate predicts cardiovascular mortality in patients with Type II diabetes. AGP can be used as a marker for inflammation, chronic alcohol drinking, chronic kidney disease, and asthma.
ab108854 employs a quantitative competitive enzyme immunoassay technique that measures Human plasma and serum Alpha 1 acid Glycoprotein (AGP) in less than 3 hours. A polyconal antibody specific for Human AGP has been pre-coated onto a 96-well microplate with removable strips. AGP in standards and samples is competed by a biotinylated AGP sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsCompetitive ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated AGP 1 vial Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human AGP Microplate 1 unit Human AGP Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
- Research Areas
modificationsN-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).
- A1AG1_HUMANAGP 1AGP-A
- AGP1Alpha-1-acid glycoprotein 1OMD 1ORMORM1orosomucoid 1Orosomucoid-1
Our Abpromise guarantee covers the use of ab108854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit images
Example of standard curve obtained with ab108854.
References for Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit (ab108854)
ab108854 has not yet been referenced specifically in any publications.