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Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit (ab108854)

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Overview

  • Product nameAlpha 1 acid Glycoprotein (AGP) Human ELISA KitSee all Alpha 1 acid Glycoprotein kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.06 µg/ml
  • Range
    0.063 µg/ml - 4 µg/ml
  • Recovery

    = 98.5 %

  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Alpha 1 acid Glycoprotein (AGP) is an acute-phase protein secreted by the liver which under conditions of inflammation increase several-fold in concentration. An elevated serum level of acute-phase inflammatory markers is associated with an increased risk of cardiovascular disease Urinary orosomucoid excretion rate predicts cardiovascular mortality in patients with Type II diabetes. AGP can be used as a marker for inflammation, chronic alcohol drinking, chronic kidney disease, and asthma.

    ab108854 employs a quantitative competitive enzyme immunoassay technique that measures Human plasma and serum Alpha 1 acid Glycoprotein (AGP) in less than 3 hours. A polyconal antibody specific for Human AGP has been pre-coated onto a 96-well microplate with removable strips. AGP in standards and samples is competed by a biotinylated AGP sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsCompetitive ELISA more details

Properties

  • FunctionFunctions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • DomainContains a beta-barrel that binds various ligands in its interior.
  • Post-translational
    modifications    N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        A1AG1_HUMANAGP 1AGP-A
        AGP1Alpha-1-acid glycoprotein 1OMD 1ORMORM1orosomucoid 1Orosomucoid-1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108854 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Competitive ELISA cELISA

      Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit images

      • Competitive ELISA - Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit (ab108854)
        Competitive ELISA - Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit (ab108854)
        Example of standard curve obtained with ab108854.

      Protocols

      References for Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit (ab108854)

      ab108854 has not yet been referenced specifically in any publications.

      Publishing research using ab108854 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108854 Submit a Question for ab108854

      Displaying 1 - 4 of 4 results for Abreviews and Q&A

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      Votes: Highest First

      Our customer is interested in purchasing ab108854, Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit. He would like to know if supplier also can supply control serum samples for this test?

      Thank you for your inquiry.

      Unfortunately, we do not have any Human serum available in our catalog that could be used as control and the kit ab108854 (http://www.abcam.com/ab108854) comes with Human AGP Standard but not with control serum.
      Read More

      Thanks for that the OD from your lab is helpful.


      I think that you should put a new standard curve in your booklet (page 12).


      You say that it is on a log scale and that is why it appears not to go to 4 ug/ml however the x ...

      Read More

      Thank you for your reply.

      We will update the graph, as it indeed confusing. When +2, 1, 0, -1 and -2is written, it means actually 102, 101, 100, 10-1, 10-2 so 100, 10, 1, 0.1 and 0.01.

      Please excuse for this error. As said, I already s...

      Read More

      I have been using one of your ELISA kits:

      ALPHA 1 ACID GLYCOPROTEIN ab108854

      I have a number of questions regarding the kit:

      1.The dilution of standards that you suggest are 0.063 to 4 ug/ml (page 9). The standard curve th...

      Read More

      Thank you for contacting us and your patience while we were investigating this issue.

      I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that w...

      Read More

      Dear Sir

      Please find below a request from our customer ,, would you help her ?

      thanks



      I want the methods for storage of blood samples used for testing alpha 1 acid glycoprotein in refrigerators and the pamphlet...

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      Thank you for your inquiry.

      I can recommend ab108854 for your customer since this kit is tested and guaranteed for plasma and serum samples. ab108852 is not.

      Please find the protocol booklet for ab108854 attached.

      Plasma and ...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"