Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit (ab108854)

Overview

  • Product nameAlpha 1 acid Glycoprotein (AGP) Human ELISA KitSee all Alpha 1 acid Glycoprotein kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.1 µg/ml
  • Range
    0.063 µg/ml - 4 µg/ml
  • Recovery

    = 98.5 %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Alpha 1 acid Glycoprotein (AGP) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of Alpha 1 acid Glycoprotein in plasma and serum.

    An Alpha 1 acid Glycoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Alpha 1 acid Glycoprotein is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Alpha 1 acid Glycoprotein captured in plate.

  • Tested applicationsCompetitive ELISA more details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Biotinylated Human Alpha 1 acid Glycoprotein (Lyophilized) 1 vial
    20X Wash Buffer Concentrate 1 x 30ml
    Alpha 1 acid Glycoprotein Microplate (12 x 8 well strips) 1 unit
    Alpha 1 acid Glycoprotein Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research Areas
  • FunctionFunctions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • DomainContains a beta-barrel that binds various ligands in its interior.
  • Post-translational
    modifications
    N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P02763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • A1AG1_HUMAN
    • AGP 1
    • AGP-A
    • AGP1
    • Alpha-1-acid glycoprotein 1
    • OMD 1
    • ORM
    • ORM1
    • orosomucoid 1
    • Orosomucoid-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Competitive ELISA Use at an assay dependent dilution.

Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit images

  • Representative Standard Curve using ab108854.

     

Protocols

References for Alpha 1 acid Glycoprotein (AGP) Human ELISA Kit (ab108854)

ab108854 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

Unfortunately, we do not have any Human serum available in our catalog that could be used as control and the kit ab108854 (http://www.abcam.com/ab108854) comes with Human AGP Standard but not with control serum.
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Thank you for your reply.

We will update the graph, as it indeed confusing. When +2, 1, 0, -1 and -2is written, it means actually 102, 101, 100, 10-1, 10-2 so 100, 10, 1, 0.1 and 0.01.

Please excuse for this error. As said, I alread...

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Thank you for contacting us and your patience while we were investigating this issue.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product tha...

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Thank you for your inquiry.

I can recommend ab108854 for your customer since this kit is tested and guaranteed for plasma and serum samples. ab108852 is not.

Please find the protocol booklet for ab108854 attached.

Plasma a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"