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Alpha 1 acid Glycoprotein Human ELISA Kit (ab108852)

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Overview

  • Product nameAlpha 1 acid Glycoprotein Human ELISA KitSee all Alpha 1 acid Glycoprotein kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine
  • Assay typeSandwich
  • Sensitivity
    = 3 ng/ml
  • Range
    3.13 ng/ml - 200 ng/ml
  • Recovery

    = 97.5 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Alpha 1 acid Glycoprotein (AGP) is an acute-phase protein secreted by the liver which under conditions of inflammation increase several-fold in concentration. An elevated serum level of acute-phase inflammatory markers is associated with an increased risk of cardiovascular disease Urinary orosomucoid excretion rate predicts cardiovascular mortality in patients with Type II diabetes. Alpha 1 acid Glycoprotein can be used as a marker for inflammation, chronic alcohol drinking, chronic kidney disease, and asthma.

    ab108852 Alpha 1 acid Glycoprotein Human ELISA (Enzyme-Linked Immunosorbent Assay) kit employs a quantitative sandwich enzyme immunoassay technique that measures cell culture supernatant and urine Alpha 1 acid Glycoprotein in less than 4 hours. A polyclonal antibody specific for Alpha 1 acid Glycoprotein has been pre-coated onto a microplate. Alpha 1 acid Glycoprotein in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for Alpha 1 acid Glycoprotein, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Biotinylated Human Alpha 1 acid Glycoprotein Antibody (30x) 1 x 300µl
    Chromogen Substrate 1 x 8ml
    Diluent Concentrate (10x) 1 x 30ml
    Human Alpha 1 acid Glycoprotein Microplate 1 x 96 wells 1 unit
    Human Alpha 1 acid Glycoprotein Standard 1 unit
    Stop Solution 1 x 12ml
    Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl
    Wash Buffer Concentrate (20x) 2 x 30ml
  • Research Areas
  • FunctionFunctions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • DomainContains a beta-barrel that binds various ligands in its interior.
  • Post-translational
    modifications    N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        A1AG1_HUMANAGP 1AGP-A
        AGP1Alpha-1-acid glycoprotein 1OMD 1ORMORM1orosomucoid 1Orosomucoid-1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108852 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Alpha 1 acid Glycoprotein Human ELISA Kit images

      • Sandwich ELISA - Alpha 1 acid Glycoprotein Human ELISA Kit- 1 x 96 Well Plate (ab108852)
        Sandwich ELISA - Alpha 1 acid Glycoprotein Human ELISA Kit- 1 x 96 Well Plate (ab108852)

      Protocols

      References for Alpha 1 acid Glycoprotein Human ELISA Kit (ab108852)

      ab108852 has not yet been referenced specifically in any publications.

      Publishing research using ab108852 ? Please let us know so that we can cite the reference in this datasheet

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      Please find below a request from our customer ,, would you help her ?

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      I want the methods for storage of blood samples used for testing alpha 1 acid glycoprotein in refrigerators and the pamphlet...

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      Thank you for your inquiry.

      I can recommend ab108854 for your customer since this kit is tested and guaranteed for plasma and serum samples. ab108852 is not.

      Please find the protocol booklet for ab108854 attached.

      Plasma and ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"