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Overview

  • Product nameAlpha 1 microglobulin proteinSee all Alpha 1 microglobulin proteins and peptides ...
  • Protein descriptionFull length native Human Alpha 1 Microglobulin (amino acids 20-203) purified from Urine from patients with chronic renal tubular proteinuria; MWt 30kDa (UniProt P02760).
  • Uniprot accessionP02760
  • Molecular weight30kDa
  • Protein length184 amino acids
  • Expression hostNative

    • Properties

  • Purification notesNominally between 40-90% of the total protein is Alpha 1 Microglobulin.
  • FormLyophilised:Reconstitution in phosphate buffer, pH >7.0 containing 0.15M NaCl is recommended.
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: 0.02M Ammonium bicarbonate
    (May contain traces of buffer salts)
  • Concentration information loading...
  • Additional notesStarting material tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab96148 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    RID RID: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        A1MAlpha 1 microglobulin/bikunin precursorAlpha 1 microglycoprotein
        Alpha-1 microglycoproteinAlpha-1-microglobulinAMBPAMBP_HUMANBikuninComplex-forming glycoprotein heterogeneous in chargeEDC1Growth inhibiting protein 19HCPHI-30HI30IATILInter-alpha-trypsin inhibitor light chainITIITI-LCITILMicroglobulin (alpha 1)Protein AMBPProtein HCTrypstatinUristatinUronic-acid-rich proteinUTI
      see all
  • FunctionInter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
    Trypstatin is a trypsin inhibitor.
  • Tissue specificityExpressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
  • Sequence similaritiesIn the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
    Contains 2 BPTI/Kunitz inhibitor domains.
  • Post-translational
    modifications    The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
    Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02760 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Alpha 1 microglobulin protein (ab96148)

    ab96148 has not yet been referenced specifically in any publications.

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