Alpha 2 HS Glycoprotein Human ELISA Kit (ab108855)
Overview
- Product nameAlpha 2 HS Glycoprotein Human ELISA Kit
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeSerum, Plasma, Cell culture extracts
- Assay typeSandwich
- Sensitivity= 3 ng/ml
- Range3.13 ng/ml - 200 ng/ml
- Recovery
= 101 %
- Assay time4h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
The alpha-2-Heremans-Schmid Glycoprotein (AHSG), also known as alpha-2-HS-Glycoprotein, or fetuin-A, is a highly glycosylated plasma protein synthesized in liver and enriched in bone. AHSG is an abundant serum protein with powerful calcification inhibitory properties. AHSG deficiency was recently linked to cardiovascular mortality in dialysis patients. While increased fetuin-A levels positively correlated with vascular calcification in patients with diabetes and mild to moderate renal impairment, an inverse relationship was observed in dialysis patients. Both chronic inflammation and uremia may contribute to exhausting fetuin-A release in the late stages of kidney disease. It has been recently reported AHSG to be decreased in the cerebrospinal fluid of patients with Alzheimer's disease.
ab108855 is designed for detection of Human Alpha 2 HS Glycoprotein in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Alpha 2 HS Glycoprotein in less than 4 hours. A polyclonal antibody specific for Alpha 2 HS Glycoprotein has been pre-coated onto a microplate. Alpha 2 HS Glycoprotein in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Alpha 2 HS Glycoprotein, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
-
Components 1 x 96 tests Alpha 2 HS Glycoprotein Standard 1 unit Biotinylated Alpha 2 HS Glycoprotein Antibody (100x) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Alpha 2 HS Glycoprotein Microplate 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml - Research Areas
- SwissProt: P01023 Human
Applications
Our Abpromise guarantee covers the use of ab108855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Alpha 2 HS Glycoprotein Human ELISA Kit images
-
Sandwich ELISA - Alpha 2 HS Glycoprotein Human ELISA Kit (ab108855)Example of Standard curve obtained using ab108855
Protocols
References for Alpha 2 HS Glycoprotein Human ELISA Kit (ab108855)
ab108855 has not yet been referenced specifically in any publications.
