Overview

  • Product name
    Anti-Alpha B Crystallin antibody
    See all Alpha B Crystallin primary antibodies
  • Description
    Rabbit polyclonal to Alpha B Crystallin
  • Specificity
    This antibody does not cross-react with beta Crystallin nor alpha A Crystallin.
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human, Pig
    Predicted to work with: Rabbit, Hamster
  • Immunogen

    Synthetic peptide:

    REEKPAVTAAPKK

    conjugated to KLH, corresponding to amino acids 163-175 of Human alpha B Crystallin.

  • General notes
    For maximum product recovery, after thawing, centrifuge the product vial before removing cap

Properties

Applications

Our Abpromise guarantee covers the use of ab13497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
ICC 1/200.
IP Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    May contribute to the transparency and refractive index of the lens.
  • Tissue specificity
    Lens as well as other tissues.
  • Involvement in disease
    Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
  • Sequence similarities
    Belongs to the small heat shock protein (HSP20) family.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • AACRYA antibody
    • Alpha B crystallin antibody
    • Alpha crystallin B chain antibody
    • Alpha(B)-crystallin antibody
    • Alpha-crystallin B chain antibody
    • CRYA2 antibody
    • Cryab antibody
    • CRYAB_HUMAN antibody
    • Crystallin alpha B antibody
    • Crystallin alpha polypeptide 2 antibody
    • CTPP2 antibody
    • Heat shock 20 kD like protein antibody
    • Heat shock protein beta 5 antibody
    • Heat shock protein beta-5 antibody
    • HspB5 antibody
    • Renal carcinoma antigen NY REN 27 antibody
    • Renal carcinoma antigen NY-REN-27 antibody
    • Rosenthal fiber component antibody
    see all

Images

  • Alpha B Crystallin was immunoprecipitated using 0.5mg Mouse Spinal Cord tissue, 5µg of Rabbit polyclonal to Alpha B Crystallin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Spinal Cord tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13497.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 22kDa; Alpha B Crystallin

  • Anti-Alpha B Crystallin antibody (ab13497) at 1/1000 dilution + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 20 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:

See all 11 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19)
Gel Running Conditions
Reduced Denaturing (4-12% bolt gel)
Loading amount
15 µg
Treatment
125 ug/ml cigarette smoke extract, 12 hr
Specification
ARPE-19
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Apr 26 2017

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (15% Polyacrylamide)
Sample
Pig Tissue lysate - other (Muscle Sarcoplasm)
Specification
Muscle Sarcoplasm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Shannon Cruzen

Verified customer

Submitted Sep 26 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (adipocytes)
Loading amount
5 µg
Specification
adipocytes
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult kidney)
Specification
adult kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 21 2008

Thank you for your enquiry. I can confirm that other than clotting being removed from whole blood (standard serum processing), there has been no additional purification to this product. I hope this information will be useful to you. Should you ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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