Anti-Alpha Fodrin antibody (ab74311)

Overview

• Product nameAnti-Alpha Fodrin antibodySee all Alpha Fodrin primary antibodies ...
• Description
  Rabbit polyclonal to Alpha Fodrin
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide from a region between residues 1000 and 1050 of human Alpha Fodrin (NP_003118.1)

• Positive controlWhole cell lysate from HeLa, 293T or NIH3T3 cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Crosslinking

Applications

Target

• FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
• Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
• Sequence similaritiesBelongs to the spectrin family.
  Contains 3 EF-hand domains.
  Contains 1 SH3 domain.
  Contains 23 spectrin repeats.
• Post-translational
  modificationsPhosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
• Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.

Target information above from: UniProt accession Q13813 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6709 Human
  • Omim: 182810 Human
  • SwissProt: Q13813 Human
  • SwissProt: P16546 Mouse
  • Unigene: 372331 Human
  • Unigene: 204969 Mouse

• Alternative names
  (ALPHA)II-SPECTRIN antibodyAlpha II Spectrin antibodyAlpha-II spectrin antibody

  brain antibodyEIEE5 antibodyFLJ17738 antibodyFLJ44613 antibodyFodrin alpha chain antibodyFodrin, alpha antibodyNEAS antibodyNon erythrocytic spectrin alpha antibodynon-erythroid alpha chain antibodySPECA antibodySpectrin alpha chain antibodySpectrin alpha chain brain antibodySpectrin alpha non erythrocytic 1 antibodySpectrin antibodySpectrin non erythroid alpha chain antibodySpectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibodySpectrin, nonerythroid, alpha subunit antibodySpna2 antibodySPTA 2 antibodySPTA2 antibodySPTA2_HUMAN antibodySPTAN 1 antibodySPTAN1 antibody

  see all

Anti-Alpha Fodrin antibody images

• 

  Western blot - Alpha Fodrin antibody (ab74311)
  All lanes : Anti-Alpha Fodrin antibody (ab74311) at 0.1 µg/ml
  
  Lane 1 : Whole cell lysate from HeLa cells at 50 µg
  Lane 2 : Whole cell lysate from HeLa cells at 15 µg
  Lane 3 : Whole cell lysate from HeLa cells at 5 µg
  Lane 4 : Whole cell lysate from 293T cells at 50 µg
  Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
  
  developed using the ECL technique
  
  Predicted band size : 285 kDa
  Observed band size : 285 kDa
  
  
  Exposure time : 30 seconds