FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
Sequence similaritiesBelongs to the spectrin family. Contains 3 EF-hand domains. Contains 1 SH3 domain. Contains 23 spectrin repeats.
Post-translational modificationsPhosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Alpha Fodrin with ab74311 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Alpha Fodrin antibody (ab74311)
All lanes : Anti-Alpha Fodrin antibody (ab74311) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
developed using the ECL technique
Predicted band size : 285 kDa Observed band size : 285 kDa
Exposure time : 30 seconds
References for Anti-Alpha Fodrin antibody (ab74311)
has not yet been referenced specifically in any publications.
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