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Abcam's Alpha Ketoglutarate (alpha KG) Assay Kit provides a simple, sensitive and rapid means for quantifying alpha KG in a variety of samples. In the assay, alpha-ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The alpha-Ketoglutarate Assay Kit is useful for detecting alpha-ketoglutarate in the range of 0.01 to 10 nmoles.
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Alpha Ketoglutarate (alpha KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing alpha KG from transamination of glutamate, or through the action of glutamate dehydrogenase. Alpha KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism.
|a-KG Assay Buffer||WM||1 x 25ml|
|a-KG Converting Enzyme (lyophilized)||Purple||1 vial|
|a-KG Development Enzyme Mix (lyophilized)||Green||1 vial|
|a-KG OxiRed Probe (in DMSO)||Red||1 x 200µl|
|a-KG Standard (10 µmol, lyophilized)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab83431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"