Western blot abreview for Anti-alpha Synuclein (phospho S129) antibody [EP1536Y]

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (H4 cells)
Loading amount
5 µg
Specification
H4 cells
Gel Running Conditions
Reduced Denaturing (4-20%Tris-Glycine gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blcoking buffer

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/3000

Detection

Detection method
Licor
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
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Submitted Nov 03 2009

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