Western blot abreview for Anti-alpha Synuclein (phospho S129) antibody [EP1536Y]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% TGX gel Biorad)
Sample
Human Cell lysate - whole cell (neuronal SHY5)
Specification
neuronal SHY5
Treatment
okadaic acid for 6-8 hours 37 oC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS Tw
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG1 HRP linked
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL GE Healthcare
Bands
Specific: 19 kDa
Exposure
10 minute(s) and 0 second(s)
Negative control
Cells not treated with okadaic acid
Positive control
This was our positive control to make sure the antibody was working since the brains we used from Parkinson transgenic mice did not give any signal.

Additional data

Additional Notes
Have not published this yet but it is hard to find a good commercial phospho S129 synuclein antibody so we are very happy with this. In fact we ordered another another AbCam S129 for human IHC and it worked really well for presumed Lewy bodies.
Username

Yien Kuo

Verified customer

Submitted Aug 30 2013

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