Anti-Alpha-synuclein (phospho S129) antibody (ab42906)
Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho S129)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Alpha-synuclein (phospho S129) antibody
See all Alpha-synuclein primary antibodies -
Description
Rabbit polyclonal to Alpha-synuclein (phospho S129) -
Host species
Rabbit -
Specificity
This antibody is specific for the 15 kDa alpha Synuclein protein phosphorylated at serine 129. Immunolabelling is blocked by the phosphopeptide used as an antigen but not by the corresponding dephosphopeptide. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Cow, Dog, Non human primates -
Immunogen
Synthetic peptide corresponding to Rat Alpha-synuclein (phospho S129). Phosphopeptide corresponding to amino acid residues surrounding the phospho serine 129 of Rat alpha Synuclein.
Database link: P37840 -
Positive control
- Rat cortex lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Prepared from rabbit serum by affinity purification via sequential chromatography on phosph- and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab42906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 15 kDa.
|
Notes |
---|
WB
1/1000. Predicted molecular weight: 15 kDa. |
Target
-
Function
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
Tissue specificity
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
Involvement in disease
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
Sequence similarities
Belongs to the synuclein family. -
Domain
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
Post-translational
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
Cellular localization
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
-
Database links
- Entrez Gene: 478478 Dog
- Entrez Gene: 20617 Mouse
- Entrez Gene: 29219 Rat
- SwissProt: O55042 Mouse
- SwissProt: P37377 Rat
- Unigene: 17484 Mouse
- Unigene: 1827 Rat
-
Alternative names
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
Images
-
All lanes : Anti-Alpha-synuclein (phospho S129) antibody (ab42906) at 1/1000 dilution
Lane 1 : Rat cortex lysate at 20 µg
Lane 2 : Rat cortex lysate with immunogenic peptide
1ng
Predicted band size: 15 kDa
Western blot of rat cortex lysate showing specific labelling of the ~15kDa alpha Synuclein protein phosphorylated at serine 129, using ab42906 at a 1/1000 dilution. Immunolabeling is blocked by the phosphopeptide (peptide) used as immunogen.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab42906 has been referenced in 2 publications.
- Toba S et al. Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport. Sci Rep 7:16386 (2017). PubMed: 29180624
- Stoica G et al. Potential role of a-synuclein in neurodegeneration: studies in a rat animal model. J Neurochem 122:812-22 (2012). PubMed: 22639889