Overview
Fluorescent β2 adrenoceptor antagonist
β3-633-AN3
Properties
ab118167 was shown to antagonize the activity of the non-selective β agonist, isoprenaline, in three separate recombinant CHO cell lines expressing either the Human β1, β2 or β3 receptor and a cyclic AMP-responsive secreted placental alkaline phosphatase (SPAP) reporter gene. The cyclic AMP-induced expression of SPAP was measured under basal and forskolin-stimulated conditions.
To determine the KD for ab118167 at β1, β2 and β3 receptors, cells were treated with isoprenaline alone, or in the presence of ab118167 and the cyclic AMP-induced expression of SPAP measured. The KD was calculated from the rightward shift in the curve.
Applications
Our Abpromise guarantee covers the use of ab118167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Small Molecule Fluorescent Optical Detection | Small Molecule Fluorescent Optical Detection: Use at an assay dependent concentration. |
| Receptor Localization | Receptor Localization: Use at an assay dependent concentration. |
| Fluorescent Cell Imaging | Fluorescent Cell Imaging: Use at an assay dependent concentration. |
Alprenolol Fluorescent ligand (Red) images
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Fluorescent Cell Imaging - Alprenolol Fluorescent ligand (Red) (ab118167)ab118167 ligand binding and displacement.
Top (A,C): ab118167 ligand (100 nm) binding to two different live cell lines expressing β1 (A) or β2 (C) adrenoceptors.
Bottom (B, D): Binding blocked in the same cell lines β1 (B) or β2 (D) by the unlabelled competitor ICI118551 (1 µM).
Nuclei have been counter-stained with Hoechst.
References for Alprenolol Fluorescent ligand (Red) (ab118167)
ab118167 has not yet been referenced specifically in any publications.
