Overview

  • Product name
    Alsterpaullone
  • Description
    Potent cyclin-dependent kinase (CDK) and GSK-3β inhibitor
  • Biological description
    Potent CDK inhibitor (IC50 values are 3.5 (CDK1/cyclin B), 20 (CDK2/cyclin A) and 40 nM (CDK5/p35)). Also displays potent and selective inhibition of GSK-3β (IC50 values are 40 and 4 nM respectively). Antiproliferative effects seen in vitro. Cell-permeable.
  • Purity
    > 96%

Properties

Images

  • ab32505 staining AKT1 in SK-N-SH cells treated with alsterpaullone (ab141070), by ICC/IF. Decrease of AKT1 expression correlates with increased concentration of alsterpaullone, as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab141070 (alsterpaullone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32505 (1/200 dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Zahler S  et al. Anti-angiogenic potential of small molecular inhibitors of cyclin dependent kinases in vitro. Angiogenesis 13:239-49 (2010). Read more (PubMed: 20706783) »
  • Lahusen T  et al. Alsterpaullone, a novel cyclin-dependent kinase inhibitor, induces apoptosis by activation of caspase-9 due to perturbation in mitochondrial membrane potential. Mol Carcinog 36:183-94 (2003). Read more (PubMed: 12669310) »
  • Sandal T  et al. A novel, extraneuronal role for cyclin-dependent protein kinase 5 (CDK5): modulation of cAMP-induced apoptosis in rat leukemia cells. J Biol Chem 277:20783-93 (2002). Read more (PubMed: 11909854) »
  • Schultz C  et al. Paullones, a series of cyclin-dependent kinase inhibitors: synthesis, evaluation of CDK1/cyclin B inhibition, and in vitro antitumor activity. J Med Chem 42:2909-19 (1999). Read more (PubMed: 10425100) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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