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Western blot - Aminoadipate aminotransferase antibody (ab59132)
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Immunohistochemistry (Paraffin-embedded sections) - Aminoadipate aminotransferase antibody (ab59132)
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Product Name
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Aminoadipate aminotransferase antibody
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See all Aminoadipate aminotransferase antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Aminoadipate aminotransferase
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Immunogen
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Synthetic peptide: AGGLPNPNMF PFKTAVITVE NGKTIQFGEE MMKRALQYSP SAGIPELLSW, corresponding to amino acids 37-86 of Human Aminoadipate aminotransferase
BLAST 'AGGLPNPNMF PFKTAVITVE NGKTIQFGEE MMKRALQYSP SAGIPELLSW ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/1562500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cells; kidney.
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Cellular localization
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Mitochondrial
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Research areas
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Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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Aminoadipate aminotransferase (AADAT) is a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Aminoadipate aminotransferase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Aminoadipate aminotransferase antibody images, references, products related to ab59132 and other tools.
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Aminoadipate aminotransferase antibody images:
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 Western blot - Aminoadipate aminotransferase antibody (ab59132)
Aminoadipate aminotransferase antibody (ab59132) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 47 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Paraffin-embedded sections) - Aminoadipate aminotransferase antibody (ab59132)
ab59132 at 4-8ug/ml staining Aminoadipate aminotransferase in epithelial cells of human kidney renal tubule by Immunohistochemistry, Paraffin-embedded tissue.
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Search PubMed (MEDLINE) for references to Aminoadipate aminotransferase
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Aminoadipate aminotransferase antibody - more information
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Customer reviews (feedback) regarding Aminoadipate aminotransferase antibody |
Customer FAQs regarding Aminoadipate aminotransferase antibody |
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Aminoadipate aminotransferase antibody - related products:
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Aminoadipate aminotransferase antibody - other tools:
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