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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-Aminoadipate aminotransferase antibody
See all Aminoadipate aminotransferase products (4) ...
Description
Rabbit polyclonal to Aminoadipate aminotransferase
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Chicken, Cow, Dog
Immunogen
Synthetic peptide: AGGLPNPNMF PFKTAVITVE NGKTIQFGEE MMKRALQYSP SAGIPELLSW, corresponding to amino acids 37-86 of Human Aminoadipate aminotransferase
AGGLPNPNMF PFKTAVITVE NGKTIQFGEE MMKRALQYSP SAGIPELLSW
Positive control
HepG2 cells; kidney.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/1562500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
Tissue specificity
Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.
Pathway
Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 4/6.
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionQ8N5Z0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- 2 aminoadipate aminotransferase antibody
- 2 aminoadipate transaminase antibody
see all
Database links
- Entrez Gene: 51166 Human
- Entrez Gene: 23923 Mouse
- GenBank: NM_016228 Human
- GenBank: NM_182662 Human
- Omim: 611754 Human
- SwissProt: Q8N5Z0 Human
- SwissProt: Q9WVM8 Mouse
- Unigene: 529735 Human
see all
Anti-Aminoadipate aminotransferase antibody images:
Western blot - Aminoadipate aminotransferase antibody (ab59132)
Anti-Aminoadipate aminotransferase antibody (ab59132) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 47 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Aminoadipate aminotransferase antibody (ab59132)
ab59132 at 4-8ug/ml staining Aminoadipate aminotransferase in epithelial cells of human kidney renal tubule by Immunohistochemistry, Paraffin-embedded tissue.
References for Anti-Aminoadipate aminotransferase antibody (ab59132)
ab59132 has not yet been referenced specifically in any publications.
Publishing research using ab59132? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"