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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-Aminoadipate aminotransferase antibody
See all Aminoadipate aminotransferase products (4) ...
Description
Goat polyclonal to Aminoadipate aminotransferase
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: C-KPEDAKNPQKNTPK, corresponding to internal sequence amino acids 179-192 of Human Aminoadipate aminotransferase (NP_057312.1; NP_872603.1).
C-KPEDAKNP QKNTPK
Positive control
Human Liver lysate.
General notes
Both reported variants represent identical protein (NP_057312.1and NP_872603.1).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab77395 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Western blot - Aminoadipate aminotransferase antibody (ab77395)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
Tissue specificity
Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.
Pathway
Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 4/6.
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionQ8N5Z0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- 2 aminoadipate aminotransferase antibody
- 2 aminoadipate transaminase antibody
see all
Database links
- Entrez Gene: 51166 Human
- GenBank: NM_016228 Human
- GenBank: NM_182662 Human
- Omim: 611754 Human
- SwissProt: Q8N5Z0 Human
- Unigene: 529735 Human
Anti-Aminoadipate aminotransferase antibody images:
Western blot - Aminoadipate aminotransferase antibody (ab77395)
Anti-Aminoadipate aminotransferase antibody (ab77395) at 1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
Predicted band size : 47 kDa
Observed band size : 47 kDa
References for Anti-Aminoadipate aminotransferase antibody (ab77395)
ab77395 has not yet been referenced specifically in any publications.
Publishing research using ab77395? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"