Aminoadipate aminotransferase antibody (ab77395)

Product Name 

Aminoadipate aminotransferase antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to Aminoadipate aminotransferase

Immunogen 

Synthetic peptide: C-KPEDAKNPQKNTPK, corresponding to internal sequence amino acids 179-192 of Human Aminoadipate aminotransferase (NP_057312.1; NP_872603.1).
BLAST 'C-KPEDAKNPQKNTPK' with or

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human Liver lysate.

Cellular localization 

Mitochondrial

Research areas 

Signal Transduction >> Metabolism >> Amino Acids

Relevance 

Aminoadipate aminotransferase (AADAT) is a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab77395 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Both reported variants represent identical protein (NP_057312.1and NP_872603.1).

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