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Western blot - Aminoadipate aminotransferase antibody (ab77395)

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Product Name
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Aminoadipate aminotransferase antibody
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See all Aminoadipate aminotransferase antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Aminoadipate aminotransferase
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Immunogen
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Synthetic peptide: C-KPEDAKNPQKNTPK, corresponding to internal sequence amino acids 179-192 of Human Aminoadipate aminotransferase (NP_057312.1; NP_872603.1). BLAST 'C-KPEDAKNPQKNTPK' with or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000. WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Liver lysate.
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Cellular localization
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Mitochondrial
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Research areas
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Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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Aminoadipate aminotransferase (AADAT) is a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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ab77395 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Both reported variants represent identical protein (NP_057312.1and NP_872603.1).
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Aminoadipate aminotransferase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Aminoadipate aminotransferase antibody images, references, products related to ab77395 and other tools.
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Aminoadipate aminotransferase antibody images:
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Western blot - Aminoadipate aminotransferase antibody (ab77395)
Aminoadipate aminotransferase antibody (ab77395) at 1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
Predicted band size : 47 kDa Observed band size : 47 kDa
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Search PubMed (MEDLINE) for references to Aminoadipate aminotransferase
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Aminoadipate aminotransferase antibody - more information
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