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Anti-Aminoadipate aminotransferase antibody
See all Aminoadipate aminotransferase products (4) ...
Goat polyclonal to Aminoadipate aminotransferase
ELISA, WBmore details
Reacts with
Human
Synthetic peptide: C-KPEDAKNPQKNTPK, corresponding to internal sequence amino acids 179-192 of Human Aminoadipate aminotransferase (NP_057312.1; NP_872603.1).
C-KPEDAKNP QKNTPK
Human Liver lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
ab77395 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Polyclonal
IgG
Western blot - Aminoadipate aminotransferase antibody (ab77395)
(enlarge)
Our Abpromise guarantee covers the use of ab77395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.
Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 4/6.
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Mitochondrion.
Target information above from: UniProt accessionQ8N5Z0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Aminoadipate aminotransferase antibody (ab77395)

Anti-Aminoadipate aminotransferase antibody (ab77395) at 1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
Predicted band size : 47 kDa
Observed band size : 47 kDa
ab77395 has not yet been referenced specifically in any publications.
Publishing research using ab77395? Please let us know so that we can cite the reference in this datasheet
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