Overview
- Product nameAnti-Aminomethyltransferase antibodySee all Aminomethyltransferase primary antibodies ...
- DescriptionMouse polyclonal to Aminomethyltransferase
- Tested applicationsWB more details
- Species reactivityReacts with: Human
- Immunogen
Full length Aminomethyltransferase protein (Human)
- Positive controlAminomethyltransferase transfected 293T cell lysate and human kidney tissue lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab67998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 29, 30, 40 kDa (predicted molecular weight: 44 kDa). |
Target
- RelevanceThe function of this protein, blonging to the glycine cleavage system, is to catalyze the degradation of glycine. Defects in AMT are a cause of non-ketotic hyperglycinemia (NKH); also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms.
- Cellular localizationMitochondrion
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Database links
- Entrez Gene: 275 Human
- Entrez Gene: 275 Human
- Omim: 238310 Human
- SwissProt: P48728 Human
- SwissProt: P48728 Human
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Alternative names
- Aminomethyltransferase (glycine cleavage system protein T) antibodyGCE antibodyGCST antibody
- GCVT antibodyNKH antibody
see all
Anti-Aminomethyltransferase antibody images
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Western blot - Aminomethyltransferase antibody (ab67998)Anti-Aminomethyltransferase antibody (ab67998) at 1/500 dilution + human kidney tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 44 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?) -
Western blot - Aminomethyltransferase antibody (ab67998)All lanes : Anti-Aminomethyltransferase antibody (ab67998) at 1/500 dilution
Lane 1 : Aminomethyltransferase transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 44 kDa
Observed band size : 29,30 kDa (why is the actual band size different from the predicted?)
References for Anti-Aminomethyltransferase antibody (ab67998)
ab67998 has not yet been referenced specifically in any publications.
