Anti-AP-A antibody (ab109775)
Key features and details
- Rabbit polyclonal to AP-A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-AP-A antibody
See all AP-A primary antibodies -
Description
Rabbit polyclonal to AP-A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human AP-A (C terminal).
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Positive control
- HepG2 and HeLa whole cell lysate; Human Brain (cerebellum) and Human Kidney tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.00184% Tris glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 109 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 109 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells. -
Tissue specificity
Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues. -
Sequence similarities
Belongs to the peptidase M1 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 504350 Cow
- Entrez Gene: 2028 Human
- Entrez Gene: 13809 Mouse
- Entrez Gene: 397080 Pig
- Entrez Gene: 64017 Rat
- Omim: 138297 Human
- SwissProt: Q32LQ0 Cow
- SwissProt: Q07075 Human
see all -
Alternative names
- Aminopeptidase A antibody
- AMPE_HUMAN antibody
- AP-A antibody
see all
Images
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All lanes : Anti-AP-A antibody (ab109775) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 109 kDa
7.5% SDS PAGE -
ab109775, at 10 µg/ml, staining AP-A in formalin-fixed, paraffin-embedded Human Kidney tissue by Immunohistochemistry.
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ab109775, at 10 µg/ml, staining AP- A in formalin-fixed, paraffin-embedded Human Brain, cerebellum by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab109775 has been referenced in 1 publication.
- Bertucci JI et al. Ghrelin and NUCB2/Nesfatin-1 Co-Localization With Digestive Enzymes in the Intestine of Pejerrey (Odontesthes bonariensis). Anat Rec (Hoboken) 302:973-982 (2019). PubMed: 30378275