Overview
Fluorescent H2 antagonist
H2-633-AN
Excitation wavelength: 633 nm
Emission wavelength: 650 nm
Properties
To determine the KD for ab118175 at histamine H2 and H3 receptors, cells were treated with concentrations of histamine agonist alone, or in the presence of 1 µM ab118175, and the cyclic AMP-induced expression of SPAP measured.
The KD at H2 was calculated from the shift of the agonist response curve in the presence of ab118175, compared to the response curve for the agonist alone.
Applications
Our Abpromise guarantee covers the use of ab118175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Small Molecule Fluorescent Optical Detection | Small Molecule Fluorescent Optical Detection: Use at an assay dependent concentration. |
| Receptor Localization | Receptor Localization: Use at an assay dependent concentration. |
| Fluorescent Cell Imaging | Fluorescent Cell Imaging: Use at an assay dependent concentration. |
Aminopotentidine Fluorescent ligand (Red) images
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Receptor Localization - Aminopotentidine Fluorescent ligand (Red) (ab118175)Left: ab118175 ligand (30 nM) binding to CHO cells expressing histamine H2 receptors. Right: Binding blocked by the unlabelled competitor ranitidine (10 µM). Nuclei have been counter-stained with Hoechst. -
Small Molecule Fluorescent Optical Detection - Aminopotentidine Fluorescent ligand (Red) (ab118175)To determine the apparent KD for ab118175 at histamine H2 receptor, cells were treated with varying concentrations of histamine agonist alone, or in the presence of 1 µM ab118175, and the cyclic AMP-induced expression of SPAP measured.
The apparent KD at H2 was calculated from the rightward shift of the agonist response curve in the presence of ab118175, compared to the response curve for the agonist alone. KD value for H2 was found to be 8.94 Antagonist -log KD. -
Small Molecule Fluorescent Optical Detection - Aminopotentidine Fluorescent ligand (Red) (ab118175)To determine the apparent KD for ab118175 at histamine H3 receptor, cells were treated with varying concentrations of histamine agonist alone, or in the presence of 1 µM ab118175, and the cyclic AMP-induced expression of SPAP measured.
The apparent KD at H3 was calculated from the rightward shift of the agonist response curve in the presence of ab118175, compared to the response curve for the agonist alone. The KD for ab118175 was found to be 7.3 Antagonist-log KD
References for Aminopotentidine Fluorescent ligand (Red) (ab118175)
ab118175 has not yet been referenced specifically in any publications.
