Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509)

Overview

  • Product nameAmmonia Assay Kit - Modified Berthelot - (Colorimetric)
    See all Ammonia kits
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    < 10 µM
  • Assay time
    0h 30m
  • Product overview

    Abcam provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (λ=670nm) using a plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The kit can detect less than 1 nmol(~10 µM) ammonia or ammonium using either a 96 well or 384 well microwell plate.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceAmmonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.

Applications

Our Abpromise guarantee covers the use of ab102509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Ammonia Assay Kit - Modified Berthelot - (Colorimetric) images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted.

  • Ammonia measured in biological fluids showing quantity (mM) in tested samples.

  • Standard curve generated in standard flat-bottom 96 well plates

Protocols

References for Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509)

ab102509 has not yet been referenced specifically in any publications.

Product Wall

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In response to your questions on this kit, the pH of the sample should be ˜7-8. To use mouse stool sample, add assay buffer to sample. Mix it completely either by pipetting up & down, sonicating or vortexing. Centr...

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Thank you for contacting us. I have spoken with the lab regarding the need to deproteinize mouse urine samples with this kit. They would still strongly recommned this step to attain the best possible data with this product. I hope this informatio...

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This product may be used to measure ammonium. In thisassay, the standards used are ammonium. Adjustment of the protocol is not required and detection levels will be the same.


I hope this infor...

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The most common reason for a low standard curve is a mistake when preparing the dilutions. I can tell from my on personal experience that a 10 times calculation mistake is especially easy to make.

Therefo...

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I have talked to the lab and the kit components are stable for at least 6 months (and up to 12 month) from the shipping date, if stored at the right temp and conditions.

Please let me know i...

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Thank you for contacting us. The kits you listed are appropriate for your samples, though we recommend ab83360 over ab102509 for assay of ammonia in urine samples. None have been published, to our knowledge. For a quote, we will need a shippin...

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Thank you for your inquiry. The lab confirmed that yes, these assay kits are compatible with serum samples from mammalian samples but they did not provide an exact protocol. I've included our general serum sample protocol below: For serum: Coll...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"