Ammonia Assay Kit (ab83360)
- Product nameAmmonia Assay KitSee all Ammonia kits ...
- Tests100 x 1 assay
- Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
- Assay typeQuantitative
- Sensitivity20 µM
- Assay time1h 00m
- Product overview
Abcam's Ammonia Assay Kit provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In the assay, Ammonia is converted to a product that reacts with the OxiRed probe to generate color (λmax = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 µM) of ammonia, much more sensitive than measuring NADPH based ammonia assay.
Visit our FAQs page for tips and troubleshooting.
Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Ammonia Assay Buffer WM 1 x 25ml Converting Enzyme (Lyophilized) Blue 1 vial Developer (Lyophilised) Orange 1 vial Enzyme Mix (lyophilized) Green 1 vial NH4Cl Standard Yellow 1 x 100µl OxiRed Probe in DMSO Red 1 x 200µl
- Research Areas
Our Abpromise guarantee covers the use of ab83360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Ammonia Assay Kit (ab83360)
ab83360 has not yet been referenced specifically in any publications.