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Overview

  • Product nameAmmonia Assay KitSee all Ammonia kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    20 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's Ammonia Assay Kit provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In the assay, Ammonia is converted to a product that reacts with the OxiRed probe to generate color (λmax = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 µM) of ammonia, much more sensitive than measuring NADPH based ammonia assay.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceAmmonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.

    • Applications

    Our Abpromise guarantee covers the use of ab83360 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Ammonia Assay Kit images

    • Functional Studies - Ammonia Assay Kit (ab83360)
      Functional Studies - Ammonia Assay Kit (ab83360)
      Example of ammonia standard curve using ab83360

    Protocols

    References for Ammonia Assay Kit (ab83360)

    ab83360 has not yet been referenced specifically in any publications.

    Publishing research using ab83360 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab83360 Submit a Question for ab83360

    Displaying 1 - 10 of 24 results for Abreviews and Q&A

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    I have order two Abcam kits to measure beta-HB and Ammonia in mouse serum sample--beta Hydroxybutyrate (beta HB) Assay Kit (ab83390) and Ammonia Assay Kit (ab83360). Do you have any special suggestions on how to prepare blood sample?

    For serum or plasma samples, there are no special requirements for preparation of serum or plasma samples for the ammonia assay. For the beta HB assay, it is recommended (see section 4 of the protocol) that samples be de-proteinated with a 10 kDa filter, ...

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    1. Is pH of the sample an important consideration when using this kit?
    2. Can you recommend a protocol for preparing mouse stool samples for use with this kit?

    Thank you for contacting us.
    In response to your questions on this kit, the pH of the sample should be ˜7-8. To use mouse stool sample, add assay buffer to sample. Mix it completely either by pipetting up & down, sonicating or vortexing. Centrifu...

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    We purchased ab83360 lot GR89403-1 (also have GR89403-3, but have not yet used). We are having issues with the standard curve. The protocol and procedures have been directly followed. Here is an example of our OD at 570: 0.188, 0.357, 0.409, 0.530, 0.686,...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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    Thank you for the email. The PO numbers for the kits are xxx and xxx. At this moment we do not have anything to purchase but if you can credit it for future that will be great. Thank you.

    Thank you for your email.

    Your credit note IDs are xxx and xxx.

    I am sorry that these kits did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed again...

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    We are having issues with the standard curve. The protocol and procedures have been directly followed. Here is an example of our OD at 570: 0.188, 0.357, 0.409, 0.530, 0.686, 0.781 for 0, 2, 4, 6, 8, and 10nmol, respectively. R2=0.9869. This is the best d...

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    Thanks for your enquiry and for providing the standard curve data.
    The standard curve data you provided look good and are quite similar to the example standard curve seen on the online product datasheet for this product. If you could, please further ...

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    Thank you for the information and your willingness to refund us. Is there a way I can get other products worth the same price instead of getting refunded? We actually purchased at 5-6 kits from abcam and did several different experiments. Some of which (c...

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    Thank you for your patience.

    I have checked with the lab about more details about the kit's mechanism. Thefollowingdetails are the ones they can give out:

    Our assay buffer contains some ingredients which can interact with the ammonia/...

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    Thank you for the information and your willingness to refund us. Is there a way I can get other products worth the same price instead of getting refunded? We actually purchased at 5-6 kits from abcam and did several different experiments. Some of which (c...

    Read More

    Thank you for your reply.

    Yes, I had offered in my email to either replace or credit/ refund those kits that did not work. You had mentioned 2 kits that were giving you high readings for a blank. But others before had worked fine - as far as I ...

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    what tissue was the kit tested on?
    lot GR89403-4 not working, standard curve incorrect, OD reading for zero-value very high
    human samples (patients with cirrhosis -plasma, should have high ammonia)
    no change in sample prep

    Thank you for contacting us.

    The supplying lab let me know that they did not find any information on their in-house use of this kit with tissue samples. I'm checking with them where the tissue information was obtained from. I'm very sorry to not...

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    what tissue was the kit tested on?
    lot GR89403-4 not working, standard curve incorrect, OD reading for zero-value very high
    human samples (patients with cirrhosis -plasma, shoul dhave high ammonia)
    no change in sample prep

    Thank you for contacting us.

    I am still in touch with the lab regarding the tested tissue samples and will let you know as soon as I hear from them.
    In the meantime, the lab also wanted to know the following, if you would be so kind to prov...

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    Inquiry: Ammonia assay kit. I will be collected my samples in twos and threes. It would be inefficient to use the kit until I have enough samples for a whole plate. Can I aliquot and store my serum samples at -80 C until then?

    Thank you for contacting us.

    Yes, storage at -80 C until you are ready to perform the assay will be fine.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Read More

    

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"