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Overview

  • Product nameAmphiregulin Human ELISA KitSee all Amphiregulin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    16.46 pg/ml - 4000 pg/ml
  • Recovery

    83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.4 89% - 111%
    Serum 85 74% - 95%
    Plasma 72.7 64% - 87%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab99975 Amphiregulin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Amphiregulin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    500X HRP-Streptavidin Concentrate Item G 1 x 200µl
    AR Microplate (12 strips x 8 wells) coated with antihuman AR 1 unit
    Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml
    Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml
    Detection Antibody AR: biotinylated anti-human AR 2 vials
    Human Amphiregulin Standard (recombinant) 2 vials
    Standards: recombinant human AR 2 vials
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • RelevanceThe mature secreted form of amphiregulin is an 84-amino acid residue glycosylated polypeptide which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR; these are EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. Amphiregulin expression is induced by phorbol ester, estrogen, androgen, and other EGFR ligands. In vitro, amphiregulin functions as an autocrine growth stimulator to drive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. Amphiregulin is important in the development of human placenta and murine mammary gland. It is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.
  • Cellular localizationCell Membrane
    • Alternative names
        A REG ARAREG
        Colorectum cell derived growth factor CRDGFMGC13647OTTHUMP00000219184Schwannoma derived growth factorSDGF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99975 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Amphiregulin Human ELISA Kit images

      • ELISA - Amphiregulin Human ELISA Kit (ab99975)
        ELISA - Amphiregulin Human ELISA Kit (ab99975)

      Protocols

      References for Amphiregulin Human ELISA Kit (ab99975)

      ab99975 has not yet been referenced specifically in any publications.

      Publishing research using ab99975 ? Please let us know so that we can cite the reference in this datasheet

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      Your kit only describes serum, plasma, cell culture supernatant etc. Id like to use this kit, but for tumor lysates (RIPA buffer based). Do you know if there would be a problem with this.



      A lot of our kits are very accommodating to many different samples types so lysates will likely work. If you decide to tests this kit with lysate samples, we recommend diluting the samples at least 5-fold with Assay Diluent B to minimize any ef...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"