Amphiregulin Human ELISA Kit (ab99975)
- Product nameAmphiregulin Human ELISA KitSee all Amphiregulin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 10 pg/ml
- Range16.46 pg/ml - 4000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 100.4 89% - 111% Serum 85 74% - 95% Plasma 72.7 64% - 87%
- Species reactivityReacts with: Human
- Product overview
ab99975 Amphiregulin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Amphiregulin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 500X HRP-Streptavidin Concentrate Item G 1 x 200µl AR Microplate (12 strips x 8 wells) coated with antihuman AR 1 unit Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody AR: biotinylated anti-human AR 2 vials Human Amphiregulin Standard (recombinant) 2 vials Standards: recombinant human AR 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- A REG ARAREG
- Colorectum cell derived growth factor CRDGFMGC13647OTTHUMP00000219184Schwannoma derived growth factorSDGF
Our Abpromise guarantee covers the use of ab99975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Amphiregulin Human ELISA Kit (ab99975)
ab99975 has not yet been referenced specifically in any publications.