Amphiregulin Mouse ELISA Kit (ab100668)
- Product nameAmphiregulin Mouse ELISA KitSee all Amphiregulin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range1.1 pg/ml - 800 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 85.25 76% - 95% Serum 130.1 120% - 138% Plasma 123.4 112% - 130%
- Species reactivityReacts with: Mouse
- Product overview
ab100668 Amphiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Amphiregulin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Amphiregulin Microplate (12 strips x 8 wells) coated with anti-mouse Amphiregulin 1 unit Amphiregulin Standard Mouse(recombinant) 2 vials Assay Diluent 5x concentrated buffer. ForStandard/Sample (serum/plasma samples/cell culture medium) diluent. 1 x 15ml Detection Antibody Amphiregulin : biotinylated anti-mouse Amphiregulin 1 vial Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- A REG ARAREG
- Colorectum cell derived growth factor CRDGFMGC13647OTTHUMP00000219184Schwannoma derived growth factorSDGF
Our Abpromise guarantee covers the use of ab100668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Amphiregulin Mouse ELISA Kit (ab100668)
ab100668 has not yet been referenced specifically in any publications.