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Overview

  • Product nameAmylase Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 0.2 mU/well
  • Assay time
    0h 40m
  • Product overview

    Abcam's α-amylase Assay uses ethylidene-pNP-G7 as the substrate. Once the substrate has been specifically cleaved by α-amylase, the smaller fragments produced can be acted upon by α-glucosidase, which causes the ultimate release of the chromophore that can then be measured at 405 nm. The assay can detect α-amylase content as low as 0.2 mU.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Amylases are enzymes that break starch down to sugar molecules. α-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker's yeast for instance). α-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. Increased enzyme levels in humans are associated with salivary trauma; mumps due to inflammation of the salivary glands, pancreatitis and renal failure. A simple, direct and automation-ready procedure for measuring α-amylase activity is, therefore, very desirable.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceAmylases are enzymes that break starch down to sugar molecules. Alpha-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker’s yeast for instance). Alpha-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. Increased enzyme levels in humans are associated with salivary trauma; mumps due to inflammation of the salivary glands, pancreatitis and renal failure. A simple, direct and automation-ready procedure for measuring alpha-amylase activity is, therefore, very desirable.

    • Applications

    Our Abpromise guarantee covers the use of ab102523 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Amylase Assay Kit images

    • Functional Studies - Amylase Assay Kit (ab102523)
      Functional Studies - Amylase Assay Kit (ab102523)
      Nitrophenol Standard Curve
    • Functional Studies - Amylase Assay Kit (ab102523)
      Functional Studies - Amylase Assay Kit (ab102523)
      Sample Test

    Protocols

    References for Amylase Assay Kit (ab102523)

    ab102523 has not yet been referenced specifically in any publications.

    Publishing research using ab102523 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab102523 Submit a Question for ab102523

    Displaying 1 - 10 of 13 results for Abreviews and Q&A

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    Dear,



    Is the kit Amylase Assay Kit (ab102523) also usable for measurements with chicken plasma?



    Thanks
    Kind regards

    Thank you for contacting us and for your interest in our products.

    I am pleased to confirm that the Amylase Assay Kit (ab102523) is suitable for the use with chicken plasma samples. Please do not hesitate to contact us in case you are experiencin...

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    Are the two kits assay buffers the same? Can tissue extraction be performed using a phosphate buffer instead


    These 2 kit buffers have different components and pH. They cannot be used interchangeably and it is not recommended to perform the extraction with PBS.

    Inquiry: Hello, I have a question regarding two kits: ab102523 (amylase) and ab102531 (trypsin). I have invertebrate tissues to analyse. I would like to extract those tissues with a phosphate buffer. Will this buffer be suitable with both kits? If so what...

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    The kit buffers would be ideal to extract your tissue. You can use the amylase assay buffer and the trypsin assay buffer.

    I have a few question about your product, Amylase Activity Assay Kit (ab102523).
    1) Do you know whether this product react with any lipases?
    2) Do you have a reaction formula about this product?
    3) What did you use as a positive control?
    4...

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    Thanks.


    We are not aware of any lipase interference in this assay.
    The reaction principle is mentioned on our datasheet for this product. The complete organic reaction is proprietary information. The same goes for the positive control i...

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    Thank you for your kind response. As a matter of fact I would like to know if the kit has any guideline or manual which directly pointed on saliva as a sample site and also I would be so grateful if you let me know about probable other previous similar re...

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    Thank you for contacting us.


    The protocol can be found by clicking the following link;

    http://www.abcam.com/ps/products/102/ab102523/documents/ab102523%20Amylase%20Assay%20Kit%20(Website).pdf

    We do not have received any fe...

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    Dear colleague
    I'm XA conducting a research on occupational stress and willing to use your kit ab102523 for assaying alpha amylase on SALIVA, but in data you've provided the saliva has not been mentioned directly as the sample site. Please let me kno...

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    Thank you for your enquiry regarding ab102523.

    I can confirm that this kit can be used for detecting Amylase in different sample types such as Cell culture supernatant, Urine, Serum, Plasma, Tissue Extracts, Cell culture media - as well as Other...

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    ab102523
    Can we use this kit on rat samples?

    Thank you for your telephone enquiry this morning.

    I can confirm that this kit has not been specifically tested on rat samples, and so regrettably there is no data to provide. However, theoretically it should work in rat and so we can guarantee...

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    Thank you for the offer. As I am going to use this kit for my plant protein which is not yet been understood and I do not know what type of antibody does it contain so, I would be grateful if you can provide me testing discount for the enzyme kit.

    Thank you for contacting us.

    Unfortunately, at this time our testing discount program only applies to primary antibodies, proteins, and ELISA kits. I am sorry I could not be more helpful, please let me know if you need any further assistance.

    Can you use frozen cell/lysate samples with this kit? If so how long can the samples be stored fo?
    Also, can I test the media that the cells were cultured in using this kit?

    Thank you for contacting Abcam.

    I have contacted the lab about using frozen samples with ab102523. If the samples were frozen appropriately (flash frozen followed by storing at -80C) so as to maintain the enzyme activity, it should be compatible...

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    I need to know whether the kits (color change) is based on product formed or product depleted?

    Thank you for your enquiry. Here are the details you requested. 1) Malate Assay Kit (ab83391): detects product formed (expected delivery date9th Jan) Step 1: Malate in sample + oxidation = product formed Step 2: Product formed + probe = color generation ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"