Amylase Assay Kit (ab102523)
- Product nameAmylase Assay Kit
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
- Assay typeQuantitative
- Sensitivity> 0.2 mU/well
- Assay time0h 40m
- Product overview
Abcam's α-amylase Assay uses ethylidene-pNP-G7 as the substrate. Once the substrate has been specifically cleaved by α-amylase, the smaller fragments produced can be acted upon by α-glucosidase, which causes the ultimate release of the chromophore that can then be measured at 405 nm. The assay can detect α-amylase content as low as 0.2 mU.
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Amylases are enzymes that break starch down to sugar molecules. α-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker's yeast for instance). α-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. Increased enzyme levels in humans are associated with salivary trauma; mumps due to inflammation of the salivary glands, pancreatitis and renal failure. A simple, direct and automation-ready procedure for measuring α-amylase activity is, therefore, very desirable.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests 1 kit Amylase Assay Buffer WM 1 x 25ml 1 x 25ml Amylase Positive Control Red 1 vial 1 vial Amylase Substrate Mix NM 1 x 5ml 1 x 5ml Nitrophenol standard Yellow 1 x 150µl 1 x 150µl
- Research Areas
Our Abpromise guarantee covers the use of ab102523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Amylase Assay Kit (ab102523)
ab102523 has not yet been referenced specifically in any publications.