Anti-Amylin antibody [R10/99] (ab11022)

Overview

• Product nameAnti-Amylin antibody [R10/99]See all Amylin primary antibodies ...
• Description
  Mouse monoclonal [R10/99] to Amylin
• SpecificityThe antibody is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid.
• Tested applicationsIHC-Fr, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cat, Dog, Chinese Hamster
• Immunogen

  Synthetic peptide: CATQRLANFLV, corresponding to Internal sequence amino acids 40-50 of Human Amylin coupled to tuberculin.

  Run BLAST with Run BLAST with
• General notesThis product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: Tissue Culture Supernatant
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberR10/99
• MyelomaSp2/0
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

Applications

Target

• FunctionSelectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
• Sequence similaritiesBelongs to the calcitonin family.
• DomainThe mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation.
• Post-translational
  modificationsAmyloid fibrils are degraded by IDE.
• Cellular localizationSecreted.

Target information above from: UniProt accession P10997 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 751513 Cat
  • Entrez Gene: 3375 Human
  • Entrez Gene: 15874 Mouse
  • Entrez Gene: 100009489 Rabbit
  • Entrez Gene: 24476 Rat
  • Omim: 147940 Human
  • SwissProt: P12967 Cat
  • SwissProt: P10997 Human

  • SwissProt: P12968 Mouse
  • SwissProt: Q07334 Rabbit
  • SwissProt: P12969 Rat
  • Unigene: 46835 Human
  • Unigene: 415 Mouse
  • Unigene: 11394 Rat

  see all

• Alternative names
  Amylin antibodyDAP antibodyDiabetes associated peptide antibody

  Diabetes-associated peptide antibodyIAP antibodyIAP antibodyIAPP antibodyIAPP_HUMAN antibodyInsulinoma amyloid peptide antibodyIslet amyloid polypeptide (diabetes associated peptide; amylin) antibodyislet amyloid polypeptide antibody

  see all

Anti-Amylin antibody [R10/99] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin antibody [ R10/99 ] (ab11022)This picture was kindly supplied as part of the review submitted by one of our customers.

  This image shows paraffin embedded mouse pancreatic islets stained with ab11022  at a dilution of 1:200, secondary stain Alexa-Fluor 488 (anti-mouse) at 1:250 dilution.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Amylin antibody [ R10/99 ] (ab11022)
  ab11022, at a dilution of 1/100, staining Amylin in human pancreas section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). It shows labeling of Islet of Langerhans.