The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/5000.
WB: 1/3000. Detects a band of approximately 45 kDa.
IHC labels dystrophic neurites in senile plaques and shows immunoreactivity with neurons containing the APPFM protein.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceAmyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimers disease patients and Downs Syndrome patients.
Cellular localizationCell Membrane, Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus