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Synthetic peptide conjugated to KLH, corresponding to amino acids 1-17 of the mature Human Amyloid Precursor Protein
Our Abpromise guarantee covers the use of ab12266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50 - 1/500.|
|ICC||Use at an assay dependent concentration. Use ice-cold methanol fixation for 10 minutes, followed by a brief wash with TBS. We have also used 4% paraformaldehyde for 20 minutes followed by permeabilization with either methanol of 0.5% Triton X-100 in TBS and this also seems to work well.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol. Please note: it is important if ab12266 is being used to stain amyloid plaques in Alzheimer's disease brains that the sections are pre-treated with formic acid. This requirement applies to most, if not all amyloid antibodies and is not unique to this product.|
Immunoprecipitation and immunoblotting of soluble APP, resulting from the action of alpha-secretase on membrane-bound APP.
Lane 1: monkey brain soluble fraction
Lane 2: conditioned medium from HEK 293 cells immunoprecipitated with ab12266.
After SDS-PAGE and transfer, the nitrocellulose was immunoblotted with MABDE2B4 followed by alkaline phosphatase-conjugated anti-mouse secondary antibody, then developd with BCIP/NBT chromogenic substrate.
The upper line marks APP751, the lower line marks APP695.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"