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Synthetic peptide corresponding to Human Amyloid Precursor Protein (N terminal).
Our Abpromise guarantee covers the use of ab15273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Ready-to-use for 10 minutes at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.
ab15273 staining human HEK cells (transfected with swAPP-GFP) by immunocytochemistry. The antibody was added to living cells for 1 hour before the cells were fixed and bound antibody was detected with a goat anti-mouse IgG Alexa-Fluor 568 ® antibody. In the confocal image ab15273 recognises the N-term extracellular portion of the APP at the membrane as well as internal pools of APP. The GFP was attached to the APP at the C-terminus.
This image is corutesy of an Abreview submitted by Randal Moldrich on 22 February 2006.
ab15273 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"