Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
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ab32136 has been referenced in 6 publications.
Publishing research using ab32136? Please let us know so that we can cite the reference in this datasheet
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Anti-Amyloid beta precursor protein antibody [Y188] (ab32136) at 1/20000 dilution + Hela cell lysate
Predicted band size : 87 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
Ab32136, at a 1/250 dilution, staining Amyloid beta precursor protein by immunohistochemistry.
Positive immunohistochemical staining, using paraffin embedded human brain tissue (A).
Negative immunohistochemical staining, using human breast (B), skeletal muscle (C) and liver (D) tissues.
Tissues were stained in parallel on the same Normal Tissue Array.
Overlay histogram showing PC12 cells stained with ab32136 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32136, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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