Amyloid beta precursor protein antibody [Y188] (ab32136)

Product Name 

Amyloid beta precursor protein antibody [Y188]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y188] to Amyloid beta precursor protein

Immunogen 

A synthetic peptide corresponding to residues in the NPXY motif of human Amyloid Beta A4 precusor (APP).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody specifically recognizes Amyloid beta precursor protein and its isoforms. It will not recognize either of the two Amyloid Beta protein variants, Amyloid Beta 1-40 or Amyloid Beta 1-42.

Tested applications 
(see key)

Flow Cyt, ICC, IHC-FoFr, IHC-FrFl, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/20.
ICC: 1/250 - 1/500.
IHC-P: 1/250 - 1/500.
IHC-FoFr: 1/750 (See PMID 18974297).
IP: 1/50.
WB: 1/5000 - 1/20000. Detects a band of approximately 95 kDa (predicted molecular weight: 87 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Hela cell lysate.
IHC-P: Human brain tissue.

Cellular localization 

Type I membrane protein.

Research areas 

Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid

Relevance 

Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver; ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri; moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y188

Isotype 

IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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References for Amyloid beta precursor protein antibody [Y188] (ab32136)

  This product has been used in: (two most recent references)

Mastrangelo MA et al. Interferon-{gamma} differentially affects Alzheimer's disease pathologies and induces neurogenesis in triple transgenic-AD mice. Am J Pathol 175:2076-88 (2009). FRET, IHC-FoFr; Mouse. PubMed: 19808651

Frazer ME et al. Reduced pathology and improved behavioral performance in Alzheimer's disease mice vaccinated with HSV amplicons expressing amyloid-beta and interleukin-4. Mol Ther 16:845-53 (2008). PubMed: 18388924

See all 4 publication references for this product.


If you publish research using ab32136 please let us know so that we can cite the reference on this datasheet.

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