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Synthetic peptide from within human KPI domain of human APP751/770 conjugated to Keyhole Limpet Heamocyanin (KLH)
Our Abpromise guarantee covers the use of ab12269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 116 kDa.|
|ICC||1/100 - 1/500. Requires denatured and reduced sample to allow access to epitope.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Requires denatured and reduced sample to allow access to epitope.|
Soluble extract of macaque brain (Macaca fasicularis, lanes 1, 3, 5) or conditioned medium from CHO cells expressing recombinant human APP770 (lanes 2, 4, 6) were separated by 7.5% SDS-PAGE, transferred to nitrocellulose and immunoblotted with the following antibodies:
Interpretation of Legend: DE2B4 recognises an epitope present in soluble APP cleaved by
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