Androgen Receptor antibody [AN1-15] (ab2742)

Product Name 

Androgen Receptor antibody [AN1-15]

Product type 

Primary antibodies

Description 

Rat monoclonal [AN1-15] to Androgen Receptor

Immunogen 

A fusion protein corresponding to residues 331-572 of human androgen receptor.

Reacts with 
(species key)

Hu, Ms, Rat, NHuPrm

Specificity 

Androgen receptor. This product does not cross-react with estrogen, progesterone or glucocorticoid receptors.

Tested applications 
(see key)

ICC, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: Use at a concentration of 5 µg/ml.
IHC-P: 1/10. Perform enzymatic antigen retrieval (Pronase (6mg/10 ml PBS) 9 min in 37°C water bath) before commencing with IHC staining protocol.
IHC-Fr: Use at a concentration of 4 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Reproduction >> Hormones
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Other
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Testosterone
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Testosterone

Relevance 

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the androgen receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain.

The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

AN1-15

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites fluid.

Form 

Lyophilised
Reconstitute with 100ul PBS.

Concentration 

Batch dependent within range: 2.40 - 2.40 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Androgen Receptor antibody [AN1-15] images, references, products related to ab2742 and other tools.

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References for Androgen Receptor antibody [AN1-15] (ab2742)

  This product has been used in: (two most recent references)

Hamdi MM & Mutungi G Dihydrotestosterone activates the MAPK pathway and modulates maximum isometric force through the EGF receptor in isolated intact mouse skeletal muscle fibres. J Physiol 588:511-25 (2010). WB; Mouse. PubMed: 20008468

Barone M et al. Possible involvement of androgen receptor alterations in hepatocarcinogenesis. Dig Liver Dis 41:665-70 (2009). IHC-Fr; Human. PubMed: 19201267

See all 16 publication references for this product.


If you publish research using ab2742 please let us know so that we can cite the reference on this datasheet.

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