Anti-Androgen Receptor antibody [AR 441] (ab9474)

Dear Madam/Sir,

Happy business day.

Could you pls ask question, our customer ask us this code number (ab9474), How many test work this code?

For your informatin our customer need work IHC P and our customer want know recommend dilution.







Pls reply us as soon.





Regards,

ANSWER:

Thank you for your inquiry.

We recommend to use this antibody at a dilution of 1/50in IHC-P. This is only a recommended starting dilution and may well have to be optimized by your customer according to their needs and samples.

If the recommended dilution is used and ca 50ul per slide (this depends on the size of the sample on the slide, it needs to be covered all the way) then one vial of ab9474 will be enough for 250 slides.

Our datasheet gives all the information to calculate this. Please follow this link to review the datasheet:

http://www.abcam.com/index.html?datasheet=9474 (or use the following: http://www.abcam.com/index.html?datasheet=9474).

I hope this information was helpful and wish your customer good luck with their research.

Dear Tech support

One of our customers has ordered ab9474. The customer wishes to know what are the chances of the antibody working With Mouse samples? Also, is there a positive control available for this antibody? Thanks in advance for your kind help.

Regards,

ANSWER:

Thank you for your interest.

It is very likely that ab9474 works in mouse but we have no data yet. According to Blast search, the homology between human and mouse AR is very high.

http://www.uniprot.org/blast/uniprot/2011101050RXMELAA8

Furthermore, we have some alternative antibodies against AR in our catalogue which proved to recognize mouse AR: ab2742, ab74272, ab3509, ab52615

Normal brain, epididymis, prostate, seminal vesicle; or skin and prostate cancer can be used as good positive control.

I hope this helps and if I can assist further, please do not hesitate to contact me.

We are using your mouse anti-human Androgen Receptor antibody (CAT# ab9474-250) to perform some immunohistochemistry in human testis frozen biopsy.

I am writing to ask you some technical information about the fixing procedure required for the use ofthe antibody. In particular, is it allowed to fix tissue slices in methacetic solution (methanol/acetic acid 3:1) without loosing the immunoreactivity? This is in order to perform a FISH staining and an immunofluorescence in the same sample. I thank you in advance for your cooperation and look forward for your kind answer.

ANSWER:

I can confirm that we have not used b9474 in methacetic solution fixed tissue so we do not know if this fixative is suitable for this antibody. We know that this antibody should work on frozen tissue sections using cold acetone as fixative (with an ABC/DAB detection method) and on formalin fixed paraffin embedded section(with an ABC/DAB detection method).

May I please encourage you to submit an Abreview via the online product datasheet once you have tested the antibody? We always encourage customers to send their results back to us, whether positive or negative, and make all product information available to researchers.

To find out more about our Abreview system, please see the following URL: http://www.abcam.com/abreviews

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Is the human Androgen Receptor antibody you sell neutralizing? (Is is proven to block protein function? To what extent?)

ANSWER:

All the informaiton we have for this antibody is on the datasheet, and to our knowledge, it has not been tested in a neutralising assay.