Anti-Androgen Receptor antibody (ab19066)
Overview
- Product nameAnti-Androgen Receptor antibodySee all Androgen Receptor primary antibodies ...
- DescriptionGoat polyclonal to Androgen Receptor
- Tested applicationsWB, ELISA, IHC-Pmore details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee
- Immunogen
Synthetic peptide:
EVQLGLGRVYPRPPSC
, corresponding to N terminal amino acids 2-16 of Human Androgen Receptor - Positive control
- Human Brain and Human Heart lysates.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab19066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). | |
| ELISA | 1/64000. | |
| IHC-P | Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- FunctionSteroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3.
- Tissue specificityIsoform 2 is mainly expressed in heart and skeletal muscle.
- Involvement in diseaseDefects in AR are the cause of androgen insensitivity syndrome (AIS) [MIM:300068]; previously known as testicular feminization syndrome (TFM). AIS is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Affected males have female external genitalia, female breast development, blind vagina, absent uterus and female adnexa, and abdominal or inguinal testes, despite a normal 46,XY karyotype.
Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1) [MIM:313200]; also known as Kennedy disease. SMAX1 is an X-linked recessive form of spinal muscular atrophy. Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX1 occurs only in men. Age at onset is usually in the third to fifth decade of life, but earlier involvement has been reported. It is characterized by slowly progressive limb and bulbar muscle weakness with fasciculations, muscle atrophy, and gynecomastia. The disorder is clinically similar to classic forms of autosomal spinal muscular atrophy. Note=Caused by trinucleotide CAG repeat expansion. In SMAX1 patients the number of Gln ranges from 38 to 62. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
Note=Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) [MIM:312300]; also known as Reifenstein syndrome. PAIS is characterized by hypospadias, hypogonadism, gynecomastia, genital ambiguity, normal XY karyotype, and a pedigree pattern consistent with X-linked recessive inheritance. Some patients present azoospermia or severe oligospermia without other clinical manifestations. - Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. - DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
- Post-translational
modificationsSumoylated on Lys-386 (major) and Lys-520. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-534 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. - Cellular localizationNucleus. Cytoplasm. Predominantly cytoplasmic in unliganded form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unliganded form in the presence of GNB2L1.
- Information by UniProt
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Database links
- Entrez Gene: 280675 Cow
- Entrez Gene: 367 Human
- Entrez Gene: 11835 Mouse
- Entrez Gene: 397582 Pig
- Entrez Gene: 24208 Rat
- Omim: 313700 Human
- SwissProt: P10275 Human
- SwissProt: P19091 Mouse
see all - FormThere are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
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Alternative names
- AIS antibody
- AIS antibody
- ANDR_HUMAN antibody
see all
Anti-Androgen Receptor antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody (ab19066)ab19066 (2µg/ml) staining of paraffin embedded Human Prostate following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows nuclear staining in the secretory cells of the gland.
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Western blot - Anti-Androgen Receptor antibody (ab19066)
Predicted band size : 99 kDaab19066 (0.3
ab19066 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer) approx. 99kDa. Primary incubation was 1 hour. Detected by chemiluminescence.µ g/ml) staining of Human Brain lysate (35µ g protein in RIPA buffer) approx. 99kDa. Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody (ab19066)Immunohistochemical analysis of paraffin-embedded Human prostate tissue, staining Androgen Receptor with ab19066 at 2 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).
References for Anti-Androgen Receptor antibody (ab19066)
This product has been referenced in:
- Titus MA et al. Dominant-negative androgen receptor inhibition of intracrine androgen-dependent growth of castration-recurrent prostate cancer. PLoS One 7:e30192 (2012). WB ; Human . Read more (PubMed: 22272301) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"