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Our Abpromise guarantee covers the use of ab19066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).|
|IHC-P||Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab19066 (2µg/ml) staining of paraffin embedded Human Prostate following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows nuclear staining in the secretory cells of the gland.