Anti-Androgen Receptor antibody (ab19066)

Overview

• Product nameAnti-Androgen Receptor antibody
  See all Androgen Receptor primary antibodies
• Description
  Goat polyclonal to Androgen Receptor
• Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee
• Immunogen

  Synthetic peptide:

  EVQLGLGRVYPRPPSC

  , corresponding to N terminal amino acids 2-16 of Human Androgen Receptor
  Run BLAST with Run BLAST with
• Positive control
  • Human Brain and Human Heart lysates.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• ClonalityPolyclonal
• IsotypeIgG
• Research areas
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Testosterone

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Testosterone

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Other

  • Developmental Biology
  • Reproduction
  • Hormones

Applications

Target

• FunctionSteroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
  Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
• Tissue specificityIsoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
• Involvement in diseaseAndrogen insensitivity syndrome
  Spinal and bulbar muscular atrophy X-linked 1
  Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
  Androgen insensitivity, partial
• Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.
• DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
• Post-translational
  modificationsSumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
  Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
  Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
• Cellular localizationNucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.

• Target information above from: UniProt accession P10275 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 747460 Chimpanzee
  • Entrez Gene: 403588 Dog
  • Entrez Gene: 367 Human
  • Entrez Gene: 11835 Mouse
  • Entrez Gene: 397582 Pig
  • Entrez Gene: 24208 Rat
  • Omim: 313700 Human
  • SwissProt: O97775 Chimpanzee

  • SwissProt: Q9TT90 Dog
  • SwissProt: P10275 Human
  • SwissProt: P19091 Mouse
  • SwissProt: Q9GKL7 Pig
  • SwissProt: P15207 Rat
  • Unigene: 76704 Human
  • Unigene: 39005 Mouse
  • Unigene: 394224 Mouse
  • Unigene: 439657 Mouse
  • Unigene: 9813 Rat

  see all
• FormThere are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
• Alternative names
  • AIS antibody
  • ANDR_HUMAN antibody
  • Androgen nuclear receptor variant 2 antibody

  • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
  • Androgen receptor antibody
  • androgen receptor splice variant 4b antibody
  • AR antibody
  • AR8 antibody
  • DHTR antibody
  • Dihydro testosterone receptor antibody
  • Dihydrotestosterone receptor (DHTR) antibody
  • Dihydrotestosterone receptor antibody
  • HUMARA antibody
  • HYSP1 antibody
  • KD antibody
  • Kennedy disease (KD) antibody
  • NR3C4 antibody
  • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
  • Nuclear receptor subfamily 3 group C member 4 antibody
  • SBMA antibody
  • SMAX1 antibody
  • Spinal and bulbar muscular atrophy (SBMA) antibody
  • Spinal and bulbar muscular atrophy antibody
  • Testicular Feminization (TFM) antibody
  • TFM antibody

  see all