Androgen Receptor antibody (ab3509)

Product Name 

Androgen Receptor antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Androgen Receptor

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu, Ms, Rat, NHuPrm

Specificity 

This antibody detects Androgen Receptor.
It does not detect estrogen, progesterone, or glucocorticoid receptors. Immunohistochemical staining of AR in rat brain results in intense nuclear staining.

Tested applications 
(see key)

ICC, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: Use at an assay dependent dilution.
IHC-Fr: 1/100. For fixation of frozen sections, 2% paraformaldehyde + 15% picric acid solution in phosphate buffer, pH 7.4 is recommended.
IP: Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 110 kDa in rat brain homogenate (predicted molecular weight: 110 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

rat brain

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Reproduction >> Hormones
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Other
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Testosterone
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Testosterone

Relevance 

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the androgen receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain.

The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Androgen Receptor antibody (ab3509)

  This product has been used in: (two most recent references)

Clancy AN et al. Distribution of androgen receptor-like immunoreactivity in the brains of intact and castrated male hamsters. Brain Res Bull 33:325-32 (1994). PubMed: 8293317

Clancy AN et al. Immunohistochemical labeling of androgen receptors in the brain of rat and monkey. Life Sci 50:409-17 (1992). PubMed: 1734159

See all 6 publication references for this product.


If you publish research using ab3509 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Androgen Receptor antibody

Customer FAQs regarding Androgen Receptor antibody

Protocols for Androgen Receptor antibody

Price and availability of products related to Androgen Receptor antibody

Androgen Receptor antibody - related products: