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Overview

  • Product nameAngiogenin (ANG) Human ELISA Kit
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 1.5 pg/ml
  • Range
    1.64 pg/ml - 400 pg/ml
  • Recovery

    94.7 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.37 85% - 104%
    Serum 95.95 82% - 105%
    Plasma 93.49 83% - 104%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab99970 Human Angiogenin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiogenin in serum, plasma and cell culture supernates. This assay employs an antibody specific for Human Angiogenin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiogenin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiogenin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiogenin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISA more details

Properties

  • RelevanceAngiogenin is a member of the ribonuclease superfamily with approximately 35% amino acid sequence identity with pancreatic RNase. Like other members of the ribonuclease superfamily, angiogenin is a cytotoxic agent that can eliminate cellular protein synthesis. Angiogenin causes inhibition of protein synthesis by functioning as a cytotoxic tRNA-specific RNase. Angiogenesis is the formation of blood vessels or capillaries from existing blood vessels due to specific signals. Angiogenin induces the growth of new blood vessels. It stimulates capillary and umbilical vein endothelial cells to produce diacylglycerol and secrete prostacyclin by phospholipase activation. Angiogenin exhibits both angiogenic and non-angiogenic activities and is involved in endothelial cell migration, proliferation, and differentiation. It is produced by a variety of tumor and normal cell types. Cells that express angiogenin include vascular endothelial and smooth muscle cells, fibroblasts, normal colonic epithelium, normal peripheral blood lymphocytes, lung and colonic epithelial tumor cell lines, and primary gastrointestinal adenocarcinomas. Angiogenin is also present in normal human plasma.
    • Alternative names
        ALS9ANGANG 1
        ANG IANG1ANGIAngiogenin ribonuclease RNase A family 5Epididymis luminal protein 168HEL168MGC22466MGC71966Ribonuclease 5Ribonuclease RNase A Family 5 Ribonuclease5RNASE 5RNASE4RNASE5
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99970 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Angiogenin (ANG) Human ELISA Kit images

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab99970.

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab99970.

      Protocols

      References for Angiogenin (ANG) Human ELISA Kit (ab99970)

      ab99970 has not yet been referenced specifically in any publications.

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      Our customer would like to purchase ab99970 and test with human tears.   If this sample can be tested, which assay diluent should be used? (There are A and B assay diluents in this kit).

      Thank you for contacting us. I have confirmed that Assay Diluent B would be the best diluent to use if testing tears.  Assay Diluent A is specifically optimized to simulate the serum/plasma matrix while Assay Diluent B is better suited for a variety of sa...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"