Overview
- Product nameAngiopoietin 1 Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 30 pg/ml
- Range74 pg/ml - 18000 pg/ml
- Recovery
< 95 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.25 83% - 105% Serum 94.47 81% - 103% Plasma 93.93 80% - 102% - Species reactivityReacts with: Human
- Product overview
Abcam’s Angiopoietin 1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Angiopoietin 1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for human Angiopoietin1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Angiopoietin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Notes
Angiopoietin 1 is one of the Angiopoietin (Ang) family of growth factors. It binds and activates the TIE2 receptor tyrosine kinase by inducing its tyrosine phosphorylation. It is implicated in endothelial developmental processes later and distinct from that of VEGF. The structure of angiopoietin 1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Angiopoietin 1 appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It also mediates blood vessel maturation/stability and it may play an important role in the heart early development. Angiopoietin 1 may have a potential therapeutic utility since it can be used for specifically targeting tumor vasculature or for promoting angiogenic processes in certain organs such as an ischemic heart.
Optimisation may be required with urine samples.
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 400X HRP-Streptavidin Concentrate Item G 1 x 200µl 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Angiopoietin 1 Microplate 1 unit Angiopoietin 1 Standard Human (recombinant) 2 vials Assay Diluent A 1 x 30ml Biotinylated anti-human Angiopoietin-1 Antibody 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml -
Research Areas
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Alternative names
- 1110046O21RikAGP 1AGP1
- AGPTANG 1ANG1 Angiopoietin1ANGPT 1ANGPT1KIAA0003
see all
- SwissProt: Q15389 Human
Applications
Our Abpromise guarantee covers the use of ab99972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
Angiopoietin 1 Human ELISA Kit images
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TYPICAL STANDARD CURVE - Assay Diluent BTYPICAL STANDARD CURVE - Assay Diluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed. -
TYPICAL STANDARD CURVE - Assay DIluent ATYPICAL STANDARD CURVE - Assay DIluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Protocols
References for Angiopoietin 1 Human ELISA Kit (ab99972)
ab99972 has not yet been referenced specifically in any publications.
