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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> Angiopoietin
Overview
Product name
Anti-Angiopoietin 1 antibody
See all Angiopoietin 1 products (6) ...
Description
Rabbit polyclonal to Angiopoietin 1
Specificity
This antiserum is directed against mouse angiopoietin 1 and shows no reactivity with angiopoietin 2 on mouse derived proteins. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2.
Tested applications
IHC-P, ICC/IF, WB, ICC, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: CNQRRNPENGGRRYNRIQHGQ, corresponding to N terminal amino acids 21/40 of Mouse Angiopoietin 1. (Peptide available as ab9076).
CNQRRNPENG GRRYNRIQHG Q
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02 K Phosphate, 0.15M NaCl, 0.1% Sodium Azide, pH7.2
Purity
Whole antiserum
Primary antibody notes
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody (and the ang-2 antibody, ab8452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Angiogenesis >> Growth Factors >> Angiopoietin
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Western blot - Angiopoietin 1 antibody (ab8451)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
WB: 1/500Detects a band of approximately 57 kDa.(Using this antiserum with cell supernatants may result in high background due to other components in the serum. This can be alleviated by immunoprecipitating the antibody:antigen complex before detection, or precipitation using soluble Tie2. These methods result in a clean, strong signal. Both Ang-1 and Ang-2 proteins have predicted MW of 57 kDa and appear on blots close to predicted MW. Additional bands may be seen at approx. 75 kDa which represent highly glycosylated forms of the protein.)
ICC: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
Sequence similarities
Contains 1 fibrinogen C-terminal domain.
Post-translational
modifications
Glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ15389
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AGP 1 antibody
- AGP1 antibody
- AGPT antibody
see all
Database links
- Entrez Gene: 284 Human
- Entrez Gene: 11600 Mouse
- Omim: 601667 Human
- SwissProt: Q15389 Human
- SwissProt: O08538 Mouse
- SwissProt: Q6NWV7 Mouse
- Unigene: 369675 Human
- Unigene: 309336 Mouse
Anti-Angiopoietin 1 antibody images:
Western blot - Angiopoietin 1 antibody (ab8451)
Supernatants of mouse-angiopoietin-expressing endothelial cells.
Lane 1 - wt endothelial cell
Lane 2 - mouse Ang-1 (clone 1-8) expressing cells
Lane 3 - mouse Ang-1 (clone 1-15) expressing cells
Lane 4 - mouse Ang-2 (clone 2-9) expressing cells
Marion Scharpfenecker 2002
References for Anti-Angiopoietin 1 antibody (ab8451)
This product has been referenced in:
- Kelsen Set al. Endothelin-A receptor blockade slows the progression of renal injury in experimental renovascular disease. Am J Physiol Renal Physiol 301:F218-25 (2011).Read more (PubMed: 21478482) »
- Silver KLet al. Dysregulation of angiopoietins is associated with placental malaria and low birth weight. PLoS One 5:e9481 (2010). WB; Mouse.Read more (PubMed: 20208992) »
See all 11 publications for this product
Publishing research using ab8451? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"