Angiopoietin 1 antibody (ab8451)

Product Name 

Angiopoietin 1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Angiopoietin 1

Immunogen 

Synthetic peptide: CNQRRNPENGGRRYNRIQHGQ, corresponding to N terminal amino acids 21/40 of Mouse Angiopoietin 1.
(Peptide available as ab9076).
BLAST 'CNQRRNPENGGRRYNRIQHGQ' with or

Reacts with 
(species key)

Hu, Ms

Specificity 

This antiserum is directed against mouse angiopoietin 1 and shows no reactivity with angiopoietin 2 on mouse derived proteins. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution (PMID 18691637).
IHC-P: Use at an assay dependent dilution (PMID 18691637).
IHC-Fr: Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 57 kDa.

The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in very clean and strong signal.

In addition, when the protein is precipitated using soluble Tie2, the signals are very good and strong.

Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Angiogenesis >> Growth Factors >> Angiopoietin

Relevance 

Angiopoietin 1 is one of the Angiopoietin (Ang) family of growth factors. It binds and activates the TIE2 receptor tyrosine kinase by inducing its tyrosine phosphorylation. It is implicated in endothelial developmental processes later and distinct from that of VEGF. The structure of angiopoietin-1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Angiopoietin 1 appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It also mediates blood vessel maturation/stability and it may play an important role in the heart early development.

Angiopoietin 1 may have a potential therapeutic utility since it can be used for specifically targeting tumor vasculature or for promoting angiogenic processes in certain organs such as an ischemic heart.


Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.

To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody (and the ang-2 antibody, ab8452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

0.02 K Phosphate, 0.15M NaCl, 0.1% Sodium Azide, pH7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

75.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Angiopoietin 1 antibody images, references, products related to ab8451 and other tools.

Angiopoietin 1 antibody images:

References for Angiopoietin 1 antibody (ab8451)

  This product has been used in: (two most recent references)

Kugathasan L et al. The angiopietin-1-Tie2 pathway prevents rather than promotes pulmonary arterial hypertension in transgenic mice. J Exp Med : (2009). WB; Mouse. PubMed: 19737862

Atluri P et al. Transmyocardial revascularization to enhance myocardial vasculogenesis and hemodynamic function. J Thorac Cardiovasc Surg 135:283-91, 291.e1; discussion 291 (2008). PubMed: 18242252

See all 9 publication references for this product.


If you publish research using ab8451 please let us know so that we can cite the reference on this datasheet.

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