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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> Angiopoietin
Overview
Product name
Angiopoietin 1 protein (Active)
See all Angiopoietin 1 products (4) ...
Protein description
Human Recombinant Full Length Angiopoietin 1 derived from HeLa cells with a C terminal histidine tag, 60-70 kDa. Swiss ID = Q15389.
Properties
Purity
>95% by SDS-PAGE
Purification notes
Greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Form
Lyophilised:
Reconstitution should be done in water to a concentration of 0.1-1.0mg/ml.
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 5% Trehalose, 200mM Sodium chloride, 20mM Sodium phosphate, pH 7.5
Concentration
Concentration information loading...
Sequence notes
Sequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein.
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Angiogenesis >> Growth Factors >> Angiopoietin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE
Functional Studies
Application notes
Biological activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
Sequence similarities
Contains 1 fibrinogen C-terminal domain.
Post-translational
modifications
Glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ15389
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for Angiopoietin 1 protein (Active) (ab69492)
ab69492 has not yet been referenced specifically in any publications.
Publishing research using ab69492? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"