Angiopoietin 2 (ANG2) Human ELISA Kit (ab99971)
- Product nameAngiopoietin 2 (ANG2) Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 10 pg/ml
- Range4.12 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 82.1 76% - 92% Serum 85.3 82% - 109% Plasma 96.9 95% - 116%
- Species reactivityReacts with: Human
- Product overview
ab99971 Angiopoietin 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human Angiopoietin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml 5X Assay Diluent Item E 1 x 15ml Angiopoietin 2 Microplate (12 strips x 8 wells) coated with anti-human Angiopoietin-2 1 unit Angiopoietin 2 Standard Human (recombinant) 2 vials Detection Antibody Angiopoietin-2: biotinylated anti-human Angiopoietin-2 2 vials HRP-Streptavidin Concentrate 200x concentrated HRP-conjugated streptavidin 1 x 200µl Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- AGPT2ANG 2ANG2
- Angiopoietin 2aAngiopoietin 2BAngiopoietin2 ANGPT 2 ANGPT2Tie2 ligand
- SwissProt: O15123 Human
Our Abpromise guarantee covers the use of ab99971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Angiopoietin 2 (ANG2) Human ELISA Kit (ab99971)
ab99971 has not yet been referenced specifically in any publications.