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Overview

  • Product nameAngiopoietin 2 (ANG2) Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    88 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 82.1 76% - 92%
    Serum 85.3 82% - 109%
    Plasma 96.9 95% - 116%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab99971 Angiopoietin 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human Angiopoietin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • RelevanceAngiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
    • Alternative names
        AGPT2ANG 2ANG2
        Angiopoietin 2aAngiopoietin 2BAngiopoietin2 ANGPT 2 ANGPT2Tie2 ligand
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99971 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Angiopoietin 2 (ANG2) Human ELISA Kit images

      • ELISA Typical Data
        ELISA Typical Data

        Representative standard curve using ab99971.

      • ELISA - Angiopoietin 2 Human ELISA Kit (ab99971)
        ELISA - Angiopoietin 2 Human ELISA Kit (ab99971)

      Protocols

      References for Angiopoietin 2 (ANG2) Human ELISA Kit (ab99971)

      ab99971 has not yet been referenced specifically in any publications.

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