Angiotensin Converting Enzyme 1 antibody [2E2] (ab11734)

Product Name 

Angiotensin Converting Enzyme 1 antibody [2E2]

Product type 

Primary antibodies

Description 

Mouse monoclonal [2E2] to Angiotensin Converting Enzyme 1

Immunogen 

Denatured Human kidney Angiotensin Converting Enzyme.

Reacts with 
(species key)

Hu, Ms, Rat, Cat, Cow, Dog, Gpig, Mk, Rb

Specificity 

Ab11734 recognises human Angiotensin Converting Enzyme.

Epitope 

This clone recognises denatured ACE, binding to an epitope in the C domain, and has been reported to be active in Western blotting.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-P: neat - 1/20. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/50 - 1/100. Predicted molecular weight: 170 (somatic) or 90 (germinal) kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

For immunohistology use kidney tissue (lung in rat studies).

Cellular localization 

Type I membrane protein

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
Signal Transduction >> Metabolism >> Vitamins / Minerals
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Blood >> Serum Proteins

Relevance 

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2E2

Myeloma 

x63-Ag8.653

Isotype 

IgG1

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.09% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This product should be stored undiluted.
Storage in frost free freezers is not recommended.
Should this product contain a precipitate we recommend microcentrifugation before use.

References for Angiotensin Converting Enzyme 1 antibody [2E2] (ab11734)

  This product has been used in: (two most recent references)

Singh VP et al. Activation of the intracellular renin-angiotensin system in cardiac fibroblasts by high glucose: role in extracellular matrix production. Am J Physiol Heart Circ Physiol 294:H1675-84 (2008). PubMed: 18296558

Sartrio CL et al. Mineralocorticoid receptor blockade improves vasomotor dysfunction and vascular oxidative stress early after myocardial infarction. Hypertension 50:919-25 (2007). WB; Rat. PubMed: 17846350

See all 4 publication references for this product.


If you publish research using ab11734 please let us know so that we can cite the reference on this datasheet.

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