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Anti-Angiotensin Converting Enzyme 1 antibody [3C5] (ab11737)

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Overview

Product name

Anti-Angiotensin Converting Enzyme 1 antibody [3C5]
See all Angiotensin Converting Enzyme 1 products (17) ...

Description

Mouse monoclonal [3C5] to Angiotensin Converting Enzyme 1

Specificity

ab11737 recognises human Angiotensin Converting Enzyme (ACE). We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Tested applications

IHC-P, WB, ELISAmore details

Cross reactivity

Reacts with

Mouse, Guinea pig, Cow, Cat, Dog, Human, Monkey

Immunogen

Denatured human kidney Angiotensin converting enzyme.

Epitope

The antibody recognises denatured ACE, binding to an epitope in the C domain.

Positive control

Human kidney.

General notes

This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

3C5

Myeloma

x63-Ag8.653

Isotype

IgG1

Research areas

Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
Signal Transduction >> Metabolism >> Vitamins / Minerals
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Blood >> Serum Proteins

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
IHC-P: 1/10 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
WB: 1/50 - 1/100. Predicted molecular weight: 170 (somatic) or 90 (germinal) kDa.

Is unsuitable for FACS.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Cellular localization

Cell Membrane

Alternative names

  • ACE 1 antibody
  • ACE antibody
  • ACE T antibody
  • ACE1 antibody
  • Angiotensin converting enzyme somatic isoform antibody
  • Angiotensin converting enzyme testis specific isoform antibody
  • Angiotensin I converting enzyme antibody
  • Angiotensin I converting enzyme 1 antibody
  • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
  • Carboxycathepsin antibody
  • CD 143 antibody
  • CD143 antibody
  • CD143 antigen antibody
  • DCP 1 antibody
  • DCP antibody
  • DCP1 antibody
  • Dipeptidyl carboxypeptidase 1 antibody
  • Kininase II antibody
  • MGC26566 antibody
  • MVCD3 antibody
  • Peptidase P antibody
  • Peptidyl dipeptidase A antibody
  • Testicular ECA antibody
see all

Database links

References for Anti-Angiotensin Converting Enzyme 1 antibody [3C5] (ab11737)

This product has been referenced in:

  • Balyasnikova IVet al. Monoclonal antibodies to denatured human ACE (CD 143), broad species specificity, reactivity on paraffin sections, and detection of subtle conformational changes in the C-terminal domain of ACE. Tissue Antigens 61:49-62 (2003).Read more (PubMed: 12622775) »

See 1 publication for this product

Publishing research using ab11737? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"