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Anti-Angiotensin Converting Enzyme 1 antibody [9B9] (ab77990)

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Overview

Product name

Anti-Angiotensin Converting Enzyme 1 antibody [9B9]
See all Angiotensin Converting Enzyme 1 products (17) ...

Description

Mouse monoclonal [9B9] to Angiotensin Converting Enzyme 1

Specificity

ab77990 recognises Angiotensin Converting Enzyme 1 (N terminal). No reactivity to testicular/germinal form of human Angiotensin Converting Enzyme 1 (90 kDa isoform).

Tested applications

WB, IP, ELISA, IHC-Fr, ICC, IHC (PFA fixed)more details

Cross reactivity

Reacts with

Rat, Hamster, Cat, Human, Pig, Monkey

Immunogen

Angiotensin Converting Enzyme 1 from human lung

Positive control

Rat heart myocardium three weeks after a myocardial infarction; human kidney

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: PBS, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

9B9

Isotype

IgG1

Research areas

Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
Signal Transduction >> Metabolism >> Vitamins / Minerals
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Blood >> Serum Proteins

  • Immunohistochemistry (Frozen sections) - Angiotensin Converting Enzyme 1 antibody [9B9] (ab77990)Immunohistochemistry (Frozen sections) - Angiotensin Converting Enzyme 1 antibody [9B9] (ab77990) image (enlarge)

  • Immunohistochemistry (Frozen sections) - Angiotensin Converting Enzyme 1 antibody [9B9] (ab77990)Immunohistochemistry (Frozen sections) - Angiotensin Converting Enzyme 1 antibody [9B9] (ab77990) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab77990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

  • IP

     

  • ELISA

     
  • 2 Images

    IHC-Fr

     

  • ICC

     

  • IHC (PFA fixed)

     

Application notes

ELISA: 1/1000.
ICC: 1/100. Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).
IHC-Fr: 1/1000.
IHC (4% PFA fixed): 1/1000.
IP: 1/1000 - 1/10,000.
WB: 1/100. Predicted molecular weight: 150 kDa.

Is unsuitable for IHC-P.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Cellular localization

Cell Membrane

Alternative names

  • ACE 1 antibody
  • ACE antibody
  • ACE T antibody
  • ACE1 antibody
  • Angiotensin converting enzyme somatic isoform antibody
  • Angiotensin converting enzyme testis specific isoform antibody
  • Angiotensin I converting enzyme antibody
  • Angiotensin I converting enzyme 1 antibody
  • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
  • Carboxycathepsin antibody
  • CD 143 antibody
  • CD143 antibody
  • CD143 antigen antibody
  • DCP 1 antibody
  • DCP antibody
  • DCP1 antibody
  • Dipeptidyl carboxypeptidase 1 antibody
  • Kininase II antibody
  • MGC26566 antibody
  • MVCD3 antibody
  • Peptidase P antibody
  • Peptidyl dipeptidase A antibody
  • Testicular ECA antibody
see all

Database links

Anti-Angiotensin Converting Enzyme 1 antibody [9B9] images:

  Immunohistochemistry (Frozen sections) - Angiotensin Converting Enzyme 1 antibody [9B9] (ab77990)

Immunohistochemistry (Frozen sections) - Angiotensin Converting Enzyme 1 antibody [9B9] (ab77990)

ab77990, at a 1/100 dilution, staining Angiotensin Converting Enzyme 1 in rat heart myocardium (three weeks after a myocardial infarction) by Immunohistochemistry.

  Immunohistochemistry (Frozen sections) - Angiotensin Converting Enzyme 1 antibody [9B9] (ab77990)

Immunohistochemistry (Frozen sections) - Angiotensin Converting Enzyme 1 antibody [9B9] (ab77990)

ab77990, at a 1/100 dilution, staining Angiotensin Converting Enzyme 1 in human kidney by Immunohistochemistry.

References for Anti-Angiotensin Converting Enzyme 1 antibody [9B9] (ab77990)

This product has been referenced in:

  • Oyamada Set al. Impact of acute myocardial ischemia reperfusion on the tissue and blood-borne renin-angiotensin system. Basic Res Cardiol 105:513-22 (2010). WB, IHC-P; Pig.Read more (PubMed: 20340028) »

See 1 publication for this product

Publishing research using ab77990? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"